Detection of Salmonella spp. in eggs: DNA analyses, culture techniques, and serology.

A polymerase chain reaction (PCR) method for direct detection of Salmonella spp. in whole-shell eggs is described. The method does not require isolating strains.

Reverse transcription PCR to detect enteroviruses in surface water.

We have developed a simple, fast, and efficient procedure to detect enteroviruses in water samples. Aliquots of water are subjected to two-step filtration, with the second filter containing a positively charged nylon membrane that holds back virus particles. Viruses thus adsorbed are directly lysed, and RNA is isolated by hybridization to specific oligonucleotides bound to magnetic beads.

Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with Listeria monocytogenes.

The polymerase chain reaction was used to obtain randomly-amplified polymorphic DNA (RAPD) profiles from Listeria spp. and enterobacteria. Eleven different oligonucleotides were evaluated.