Publications by authors named "P Buechler Frederiksen"
Understanding how inflammatory cytokines influence profibrogenic wound healing responses in fibroblasts is important for understanding the pathogenesis of fibrosis. TNF-α and IL-13 are key cytokines in Th1 and Th2 immune responses, respectively, while TGF-β1 is the principal pro-fibrotic mediator. We show that 12-day fibroblast culture with TNF-α or IL-13 induces fibrogenesis, marked by progressively increasing type III and VI collagen formation, and that TGF-β1 co-stimulation amplifies these effects.
View Article and Find Full Text PDFObjective: To assess the longitudinal stability of biomarker-based molecular endotypes of knee osteoarthritis (KOA) participants from APPROACH and to evaluate the consistency of findings in an independent KOA population.
Methods: Nineteen biomarkers were measured longitudinally in 295 KOA participants from the APPROACH cohort. K-means clustering was used to identify the structural damage, inflammation, and low tissue turnover endotypes at the six-, 12-, and 24-month follow-ups.
Aim: To investigate type III collagen (COL III) turnover in participants from the CANVAS Program biomarker substudy.
Methods: Biomarkers of COL III formation (PRO-C3) and COL III degradation fragments (C3M and CTX-III) were assessed in baseline and year 3 plasma from patients enrolled in CANVAS, investigating the effect of canagliflozin in participants with type 2 diabetes. The clinical outcomes investigated in this study were hospitalization for heart failure, cardiovascular death and all-cause mortality.
Fibrosis, driven by fibroblast activities, is an important contributor to morbidity and mortality in most chronic diseases. Endotrophin, a signaling molecule derived from processing of type VI collagen by highly activated fibroblasts, is involved in fibrotic tissue remodeling. Circulating levels of endotrophin have been associated with an increased risk of mortality in multiple chronic diseases.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the relationship between global longitudinal strain (LS) assessed by speckle tracking and traditional Doppler echocardiography with physiological markers of heart function derived from pressure-volume loops in pigs with left ventricular (LV) dysfunction.
- Twelve female pigs underwent a procedure that induced LV dysfunction, leading to a significant reduction in cardiac output and mixed venous saturation, while their echocardiographic measurements were conducted at various intervals.
- Results showed that as LV dysfunction progressed, both LS and LV outflow tract velocity time integral (VTI) decreased significantly and correlated strongly with stroke work, highlighting their potential as sensitive indicators of myocardial damage in heart dysfunction.