Expanded progeny cells of diffuse cytotoxic bone marrow-derived colonies.

Bone marrow-derived (colony-stimulating factor [CSF]-dependent) diffuse colonies have been shown to include colonies with cytotoxic activity. Such diffuse colonies were expanded for 6-8 weeks in liquid culture medium in the presence of pokeweed mitogen- or concanavalin A-conditioned spleen cell medium (CM). The morphology of the expanded diffuse colony cells (EDCC) was like that of early myelocytic cells.

Cytological events in allo-stimulated lymphocytes triggered by exposure to alloantigens. V. Response to disrupted target cells.

H-2b lymphocytes sensitized against H-2d alloantigens or H-2k alloantigens were triggered by incubation with disrupted or intact H-2d target cells. The triggering of the lymphoid cells was stopped by fixation with glutaraldehyde. Changes in cell size, in numerical density of the endoplasmic reticulum (ER), and the Golgi apparatus (GA) as well as in the areal density of the lysosomes and the mitochondria in the lymphoblasts during the first three hours of triggering were measured with a Hewlett Packard digitizer.