Publications by authors named "P Benveniste"

Article Synopsis
  • This study investigates the metabolic and bioenergetic changes in the spinal cord of a transgenic mouse model of Parkinson's disease (M83) that overexpresses a mutated form of alpha-synuclein, comparing it to non-transgenic mice.!* -
  • Using advanced imaging techniques, the researchers found that the M83 mice had lower oxygen saturation levels in their spinal cords, but there were no significant changes in spinal cord volume or vascular organization despite the presence of phosphorylated alpha-synuclein.!* -
  • The study highlights the development of deep learning tools for analyzing spinal cord MRI data, and underscores the complexity of Parkinson's disease by showing reduced oxygen levels without related structural changes in the spinal cord.!
View Article and Find Full Text PDF

The differentiation of human hematopoietic stem cells into CD8 T cells can be achieved in vitro with the OP9-DL4 system. We considered that in the absence of medullary thymic epithelial cells, which serve to restrict the breath of the T-cell receptor (TCR) repertoire by expressing tissue-restricted antigens, a distinct repertoire would be generated in vitro. To test this notion, we compared the TCR-Vα/Vβ (TRAV/TRBV) gene usage of major histocompatibility complex-restricted antigen (MART-1)-specific T cells generated in vitro to that from ex vivo naïve T cells and tumor-infiltrating lymphocytes (TILs) using high-throughput DNA sequencing.

View Article and Find Full Text PDF

Antigen recognition by T cells bearing αβ T cell receptors (TCRs) is restricted by major histocompatibility complex (MHC). However, how antigens are recognized by T cells bearing γδ TCRs remains unclear. Although γδ T cells can recognize nonclassical MHC, it is generally thought that recognition of antigens is not MHC restricted.

View Article and Find Full Text PDF

Expression of transcripts for the homotypic adhesion protein epithelial V-like antigen 1 (EVA1), also known as myelin protein zero like-2 (Mpzl2), is known to be present in thymic stromal cells. However, protein expression within different thymic subsets, stromal and/or lymphoid, has not been characterized due a lack of specific reagents. To address this, we generated a hybridoma (G9P3-1) secreting a monoclonal antibody (G9P3-1Mab), reactive against both human and mouse EVA1.

View Article and Find Full Text PDF

All blood cell lineages start from hematopoietic stem cells (HSCs), which were recently shown to represent a heterogeneous group of cells. In mice, Notch signaling promotes the maintenance of "stemness" as well as the expansion of self-renewing HSCs in vitro. Additionally, human CD34(+) cells were shown to expand in vitro in response to Notch signals.

View Article and Find Full Text PDF