Publications by authors named "P Barattini"
Article Synopsis
- A research project is being conducted to create a diagnostic tool for six significant viruses affecting pigs, including ASFV, PRRSV, SIV, PPV, PCV2, and CSFV.
- A questionnaire was distributed to farmers, veterinarians, and other stakeholders in Italy, Greece, Hungary, and Poland to gauge interest in this tool, resulting in 83 responses that provided insights on disease importance, diagnostic tool challenges, and economic concerns.
- The primary users are expected to be veterinarians and pig producers, with the most interest in diagnosing PRRSV, SIV, PPV, and PCV2, and stakeholders prioritize sensitivity, cost, simplicity, and quick results, with many willing to pay up to €3,000
Article Synopsis
- The paper introduces a new diagnostic device that utilizes bio-sensing and photonics technologies for quick, on-site detection of viruses affecting pigs, helping address both emerging and ongoing epidemics.
- This device is being developed as part of a project funded by the EU Commission, aimed at enabling non-specialized personnel to perform early detection of selected swine viruses.
- It can test various biological samples like oral fluids, faeces, blood, or nasal swabs, providing immediate threat assessments and showcasing the innovative technologies involved in its design and functionality.
Increased reactive oxygen species (ROS) generation and the ensuing oxidative stress contribute to Alzheimer's disease pathology. We reported previously that amyloid-β peptide oligomers (AβOs) produce aberrant Ca(2+) signals at sublethal concentrations and decrease the expression of type-2 ryanodine receptors (RyR2), which are crucial for hippocampal synaptic plasticity and memory. Here, we investigated whether the antioxidant agent astaxanthin (ATX) protects neurons from AβOs-induced excessive mitochondrial ROS generation, NFATc4 activation, and RyR2 mRNA downregulation.
View Article and Find Full Text PDFMounting evidence indicates that iron accumulation impairs brain function. We have reported previously that addition of sub-lethal concentrations of iron to primary hippocampal neurons produces Ca(2) (+) signals and promotes cytoplasmic generation of reactive oxygen species. These Ca(2) (+) signals, which emerge within seconds after iron addition, arise mostly from Ca(2) (+) release through the redox-sensitive ryanodine receptor (RyR) channels present in the endoplasmic reticulum.
View Article and Find Full Text PDFPurpose: The aims of the present study were to better understand the role of Ku 80, which is involved in double-strand break repair in mammalian cells in the mechanism of radiation resistance and to verify the possibility of increasing cell radiosensitivity by targeted inhibition of Ku autoantigen 80 (Ku 80).
Materials And Methods: Western blot and electrophoretic mobility shift assay (EMSA) were performed on the human bladder carcinoma cell line RT112 (radioresistant) and on the human colorectal carcinoma cell line SW48 (radiosensitive) to assess the expression levels of DNA-dependent protein kinase (DNA-PK) components and the DNA-binding activity of the Ku 70/80 heterodimer after exposure to radiation, respectively. Ku 80 silencing was carried out with the use of small interfering RNA (siRNA).