Highly reduced binding to high and low affinity mouse Fc gamma receptors by L234A/L235A and N297A Fc mutations engineered into mouse IgG2a.

The effects of the Fc silencing mutations such as leucine (L) to alanine (A) substitution at the position 234 and 235 (LALA) and the alanine (A) to asparagine (N) substitution at position 297 (N297A) are well investigated for human IgG. However, the effects of the same two silencing Fc mutations in a mouse IgG backbone are not yet well investigated in respect to binding to mouse Fc gamma receptors (FcγRs), complement and subsequent effector functions. By using a mouse IgG2a tool antibody directed against mouse OX40L, we demonstrate a strongly reduced binding of the two Fc mutants to high and low affinity recombinant and cell expressed mouse FcγRs, when compared to the mouse IgG2a with the wild type (wt) backbone.

[Long-term results with the cementless CLS stem in hip replacement].

Objective: The present work aims at evaluating the clinical and radiological long-term results of the cementless CLS stem in hip replacement.

Method: Sixty-six consecutively operated patients were examined clinically and radiologically at an average follow-up time of 10.7 (7.

[Blood loss in total hip prosthesis implantation: lateral versus supine position].

We performed a prospective randomized study to determine blood loss differences between supine or lateral patient position, during surgery in elective total hip replacement. Between January and October 1996, 64 consecutive cases of total hip replacements were randomly scheduled for a procedure either in the supine or in the lateral position. Of the 56 cases evaluated, 29 were operated in the supine position (SP) and 27 in the lateral position (LP).

Radiolucent lines and component stability in knee arthroplasty. Standard versus fluoroscopically-assisted radiographs.

The radiolucent lines and the stability of the components of 66 knee arthroplasties were assessed by six orthopaedic surgeons on conventional anteroposterior and lateral radiographs and on fluoroscopic views which had been taken on the same day. The examiners were blinded as to the patients and clinical results. The interpretation of the radiographs was repeated after five months.