Assisted hatching on assisted conception (IVF & ICSI).

Background: Failure of implantation and conception may result from an inability of the blastocyst to escape from its outer coat, know as the zona pellucida. In vitro culture conditions and/or advancing maternal age may alter the architecture of the zona pellucida and result in hatching difficulties. Artificial disruption of this coat is known as assisted hatching (AH) has been proposed as a method of improving the success of assisted conception.

Assisted hatching on assisted conception (IVF & ICSI).

Background: Failure of implantation and conception may result from an inability of the blastocyst to escape from its outer coat, know as the zona pellucida. In vitro culture conditions and/or advancing maternal age may alter the architecture of the zona pellucida and result in hatching difficulties. Artificial disruption of this coat is known as assisted hatching (AH) has been proposed as a method of improving the success of assisted conception.

Effect(s) of assisted hatching on assisted conception (IVF & ICSI).

Background: Failure of implantation and thus conception might result from inability of the blastocyst to escape from its zona pellucida. Artificial disruption of this coat has been proposed as a method of improving the success of assisted conception.

Objectives: To determine whether assisted hatching of embryos facilitates live births, clinical pregnancy and implantation and whether it impacts on negative outcomes (such as miscarriage).

Cyclic modulation of epithelial glycosylation in human and baboon (Papio anubis) endometrium demonstrated by the binding of the agglutinin from Dolichos biflorus.

Glycoconjugate expression in endometrial epithelium varies in a cycle-dependent manner in many species and is probably fundamental to the establishment of a receptive uterine environment for the implanting embryo. We have examined the distribution of N-acetyl galactosamine (GalNAc)-containing sequences bound by Dolichos biflorus agglutinin (DBA) in the cycling endometrium of the human and baboon (Papio anubis). In the human, DBA binding was absent in the proliferative and early secretory phases of the cycle, increasing thereafter both in glands and on the surface epithelium.