Relationship between Hedgehog Signaling Pathway and Drug Resistance of Poorly Differentiated Gliomas.

The effects of Hedgehog signaling inhibitor (cyclopamine) and activator (Shh) on drug resistance of U251-MG human glioma cells and human astrocyte culture to cisplatin, temozolomide, and doxorubicin were studied. Cyclopamine and Shh modified the drug resistance of U251-MG cells but not of human astrocytes. Experiments with cyclopamine, Shh, and chemical drugs can contribute to detection of the mechanisms of signaling effects on the drug resistance processes, while the experimental data can serve as one of the criteria for choosing individual chemotherapy for patients.

Retinoblastoma: Magnetic Isotope Effects Might Make a Difference in the Current Anti-Cancer Research Strategy.

Human retinoblastoma cells were proven to possess some very unusual DNApolβ species. Being 23.5 kDa monomers, which itself is not common for the DNApolβ superfamily members, these chromatin associated proteins manifests most of the DNApolβ-specifc functional peculiarities making them legitimate targets for DNA repair cytostatic inhibitors.

[Diurnal cycles of endogenous phytohormones in barley].

We have studied diurnal courses of the free forms of phytohormones (abscisic acid, gibberellins, cytokinins, indolylacetic acid, and ethylene) in leaves of the spring variety of barley (Hordeum vulgare L.), cultivars Zazerskii 85 and Moskovskii 2 in vegetation experiments. The phytohormones were analyzed by gas-liquid chromatography, immunoenzyme assays, and bioassays, using unfractionated plant material.