Publications by authors named "P B Kopnin"
A growing body of evidence suggests that actin plays a role in nuclear architecture, genome organisation, and regulation. Our study of human lung adenocarcinoma cells demonstrates that the equilibrium between actin isoforms affects the composition of the nuclear lamina, which in turn influences nuclear stiffness and cellular behaviour. The downregulation of β-actin resulted in an increase in nuclear area, accompanied by a decrease in A-type lamins and an enhancement in lamin B2.
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- Resistance to hormonal and targeted therapies in breast cancer is influenced by epigenetic alterations, particularly through DNA methylation changes linked to DNMT3A suppression.
- The study investigates the role of NR6A1 as a regulatory factor involved in this suppression, finding that its downregulation is critical for developing resistance.
- The research reveals that changes in cell signaling pathways and the activation of Snail, a key regulator, are associated with resistance, suggesting that the NR6A1/DNMT3A axis could help inform future breast cancer treatment strategies.
Article Synopsis
- - This review highlights current strategies and products in gene and cell therapy for treating recessive dystrophic epidermolysis bullosa (RDEB), a disorder linked to collagen type VII alpha 1 (C7) deficiency due to genetic defects.
- - Allogenic mesenchymal stem/stromal cells show the most potential, along with genetically modified autologous dermal fibroblast injections, as key approaches in RDEB cell therapy.
- - Gene therapy methods, including gene replacement with viral vectors and gene editing, have seen significant advancements, with successful developments such as ex vivo epidermal transplants and two-layer transplants enhancing treatment options for RDEB.
Extracellular vesicles (EVs) are of growing interest in the context of screening for highly informative cancer markers. We have previously shown that uterine aspirate EVs (UA EVs) are a promising source of ovarian cancer (OC) diagnostic markers. In this study, we first conducted an integrative analysis of EV-miRNA profiles from UA, malignant ascitic fluid (AF), and a conditioned medium of cultured ascites cells (ACs).
View Article and Find Full Text PDFThe widespread occurrence of breast cancer and its propensity to develop drug resistance highlight the need for a comprehensive understanding of the molecular mechanisms involved. This study investigates the intricate pathways associated with secondary resistance to taxol in triple-negative breast cancer (TNBC) cells, with a particular focus on the changes observed in the cytoplasmic actin isoforms. By studying a taxol-resistant TNBC cell line, we revealed a shift between actin isoforms towards γ-actin predominance, accompanied by increased motility and invasive properties.
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