Amphoteric Cross-Linked Cellulose Nanoparticles as a Platform for Immobilization of Proteins and Cells, Enabling Bioanalyte Sensing.

Cellulosic nanomaterials have significantly promoted the development of sensing devices, drug delivery, and bioreactor processes. Their synthetic flexibility makes them a prominent choice for immobilizing biomolecules or cells. In this work, we developed a practical and user-friendly approach to accessing cellulose nanoparticles (CNPs).

Lattice Anisotropy-Driven Reduction of Phonon Velocities in Black Phosphorus.

Phonon dynamics and transport determine how heat is utilized and dissipated in materials. In 2D systems for optoelectronics and thermoelectrics, the impact of nanoscale material structure on phonon propagation is central to controlling thermal conduction. Here, we directly observe in-plane coherent acoustic phonon propagation in black phosphorus (BP) using ultrafast electron microscopy.

Self-reported outcomes in adults with hypospadias: A meta-analysis of patient satisfaction and quality-of-life metrics.

Introduction: Hypospadias reconstruction seeks to correct structural problems associated with this congenital condition to improve patient quality-of-life (QoL) and overall well-being. While corrective surgery can lead to major functional and psychosocial improvements, some patients experience continuing problems that require additional procedures. This study evaluates patient-reported outcomes (PROs) in hypospadias care, particularly penile satisfaction and QoL after surgery, to fill this essential gap in the literature.

Reproducible, Scale-Up Production of Human Brain Organoids (HBOs) on a Pillar Plate Platform via Spheroid Transfer.

Human brain organoids (HBOs) derived from pluripotent stem cells hold great potential for disease modeling and high-throughput compound screening, given their structural and functional resemblance to fetal brain tissues. These organoids can mimic early stages of brain development, offering a valuable in vitro model to study both normal and disordered neurodevelopment. However, current methods of generating HBOs are often low throughput and variable in organoid differentiation and involve lengthy, labor-intensive processes, limiting their broader application in both academic and industrial research.

Dynamic Culture of Bioprinted Liver Tumor Spheroids in a Pillar/Perfusion Plate for Predictive Screening of Anticancer Drugs.

Recent advancements in three-dimensional (3D) cell culture technologies, such as cell spheroids, organoids, and 3D bioprinted tissue constructs, have significantly improved the physiological relevance of in vitro models. These models better mimic tissue structure and function, closely emulating in vivo characteristics and enhancing phenotypic analysis, critical for basic research and drug screening in personalized cancer therapy. Despite their potential, current 3D cell culture platforms face technical challenges, which include user-unfriendliness in long-term dynamic cell culture, incompatibility with rapid cell encapsulation in biomimetic hydrogels, and low throughput for compound screening.