[Neonatal screening for cystic fibrosis].

Cystic fibrosis (C.F.), a congenital lethal disease involving many organs, is responsible of chronic pulmonary disease and maldigestion.

Regeneration of plants from callus tissue of the pasture legume Centrosema brasilianum.

Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.

Inosine-containing primers in human papillomavirus detection by polymerase chain reaction.

A PCR protocol has been developed using inosine-containing primers for human papilloma virus (HPV) detection. The HPV16 L1 region from CaSki-positive cells was efficiently amplified by a single reaction and directly analyzed by agarose gel electrophoresis. The method was found to be sensitive and reproducible, and it easy to use for HPV typing and screening.

Blind serosurvey of HIV antibodies in newborns in 92 Italian hospitals: a method for monitoring the infection rate in women at time of delivery.

In Italy, drug abusers are the major risk category for HIV infection, representing 67% of all the reported cases of AIDS. This can in part explain the higher incidence of the infection observed in Italian females (17.7%) in comparison with the data reported in Europe (11.

Study of the effects on DNA of electromagnetic fields using clamped homogeneous electric field gel electrophoresis.

A clamped homogeneous electric field (CHEF) electrophoresis allowing the separation of DNA molecules in the range of 200 to 3000 kb in size was used to study the biological effects of electric and magnetic fields (EMFs). The results obtained did not show any detectable genomic damage on Saccharomyces cerevisiae.