Cytotoxicity reactions against target cells infected with rabies virus.

Some aspects of the cytotoxicity reactions were studied in the rabies system. The antibody-dependent complement cytotoxicity (ADC), the cellular cytotoxicity (CC), and the antibody-dependent cellular cytotoxicity (ADCC) are shown, being the cytotoxic effect as evidenced by the 51Cr released from the cells infected with the Pasteur strain of rabies virus. Some parameters such as time of cellular infection, the amount of infected cells, the concentration of complement, and the incubation time of the ADC reaction, which help to increase the performance of this reaction, are discussed.

[Evaluation of an experimental rabies vaccine by the oral and intestinal route with inactivated vaccines, concentrated or non-concentrated].

Application of beta-propiolactone inactivated rabies vaccine prepared in bovine embryo kidney cells, concentrated or non concentrated, by intestinal or oral route resulted in antibody production in rats and cats. 80-100% of vaccinated rats were protected against challenge with street rabies virus. The same vaccines (lyophilized in gelatin capsules) stimulated antibody production in more than 50% (5/8) cats which received the vaccine by the oral route.