Method of Reduction Background Fluorescence in Human Fetal Brain Tissue and Quantitative Estimate of the Effect of Photobleaching.

We developed a method of reducing the background fluorescence of samples made from formalin-fixed and paraffin-embedded blocks of the brain of the second-trimester human fetuses. For reducing excess background fluorescence, the samples were subjected to photobleaching using an LED lamp with blue and red emission peaks in the range of visible spectrum in a construction of an original design. The decrease in the background autofluorescence was checked by measuring the intensity of the emitted background fluorescence of the samples and relative abundance of immunopositive structures after immunohistochemical staining.

Accumulation of storage proteins in plant seeds is mediated by amyloid formation.

Amyloids are protein aggregates with a highly ordered spatial structure giving them unique physicochemical properties. Different amyloids not only participate in the development of numerous incurable diseases but control vital functions in archaea, bacteria and eukarya. Plants are a poorly studied systematic group in the field of amyloid biology.

Two Novel Amyloid Proteins, RopA and RopB, from the Root Nodule Bacterium .

Amyloids represent protein fibrils with a highly ordered spatial structure, which not only cause dozens of incurable human and animal diseases but also play vital biological roles in Archaea, Bacteria, and Eukarya. Despite the fact that association of bacterial amyloids with microbial pathogenesis and infectious diseases is well known, there is a lack of information concerning the amyloids of symbiotic bacteria. In this study, using the previously developed proteomic method for screening and identification of amyloids (PSIA), we identified amyloidogenic proteins in the proteome of the root nodule bacterium Among 54 proteins identified, we selected two proteins, RopA and RopB, which are predicted to have β-barrel structure and are likely to be involved in the control of plant-microbial symbiosis.

Anthocyanin Composition and Content in Rye Plants with Different Grain Color.

The color of grain in cereals is determined mainly by anthocyanin pigments. A large level of genetic diversity for anthocyanin content and composition in the grain of different species was observed. In rye, recessive mutations in six genes (vi1.

Early Postnatal Development of the Lamination in the Lateral Geniculate Nucleus A-Layers in Cats.

The early postnatal development of the A-layers of the dorsal lateral geniculate nucleus (LGNd) was investigated in kittens aged 0-34 days by immunohistochemistry for the selective marker for neuronal differentiation (NeuN protein) and parvalbumin. We report two new facts about the LGNd development. First, there is a transient stratification of NeuN labelling in layer A, and to a lesser extent in layer A1, in kittens aged 0 and 4 days.