Expression of bovine interleukin 15 in and study on its biological activity: a T-cell activator.

Interleukin 15 (IL-15) plays crucial role as stimulatory cytokine in generation and proliferation of CD8 T memory cells. The present study was initiated to analyze the role of yeast-expressed bovine IL-15 in inducing the CD8 T memory cells. The bIL-15 was cloned in pPICZαA expression vector.

Expression and purification of biologically active bovine Interferon λ3 (IL28B) in Pichia pastoris.

Interferon lambda-3 (IFNλ3) which is also known as IL28B is a member of type III Interferons which are structurally and genetically different from type I Interferons. These Interferons induce signal transduction pathways similar to type I Interferons which results in the activation of Interferon Stimulated Genes (ISGs). This group of Interferons are tissue specific and reported to have antiviral activity.

Clinical characteristics, beta-cell dysfunction and treatment outcomes in patients with A-β+ Ketosis-Prone Diabetes (KPD): The first identified cohort amongst Asian Indians.

Objective: Ketosis-prone diabetes (KPD), an atypical form of diabetes, has emerged as a heterogeneous syndrome in multiple ethnic groups. The objectives of this study were to look into the clinical characteristics of adult Asian Indian patients with recently diagnosed, antibody negative diabetes presenting with unprovoked ketoacidosis (A-β+ KPD) and to determine the natural course of recovery of beta-cell functions on serial follow-up over one year.

Research Design And Methods: Newly diagnosed adult diabetes patients (n=11) with suspected KPD (A-β+) were prospectively studied over a period of 1-year with serial evaluations of clinical, biochemical and beta-cell secretion characteristics.