Publications by authors named "P A Nidhina Haridas"
Article Synopsis
- The study compared two surgical techniques for ACL reconstruction: the all-inside (AI) technique using hamstring autograft and the outside-in (OI) technique using an interference screw and a cortical Endobutton.
- It was a double-blinded randomized controlled trial involving 44 patients, monitored over 12 months through different scoring scales to assess pain and function.
- Results showed that the AI technique provided better outcomes and pain relief at 3, 6, and 12-month follow-ups compared to the OI technique.
Species mislabeling of commercial loliginidae squid can undermine important conservation efforts and prevent consumers from making informed decisions. A comprehensive lipidomic fingerprint of Uroteuthis singhalensis, Uroteuthis edulis, and Uroteuthis duvauceli rings was established using high-resolution mass spectrometry-based lipidomics and chemoinformatics analysis. The principal component analysis showed a clear separation of sample groups, with RX and Q values of 0.
View Article and Find Full Text PDFCommunication between adipocytes and endothelial cells (EC) is suggested to play an important role in the metabolic function of white adipose tissue. In order to generate tools to investigate in detail the physiology and communication of EC and adipocytes, a method for isolation of adipose microvascular EC from visceral adipose tissue (VAT) biopsies of subjects with obesity was developed. Moreover, mature white adipocytes were isolated from the VAT biopsies by a method adapted from a previously published Membrane aggregate adipocytes culture (MAAC) protocol.
View Article and Find Full Text PDFBackground: Thyroid hormone responsive protein (THRSP) is a lipogenic nuclear protein that is highly expressed in murine adipose tissue, but its role in humans remains unknown.
Methods: We characterized the insulin regulation of THRSP in vivo in human adipose tissue biopsies and in vitro in Simpson-Golabi-Behmel syndrome (SGBS) adipocytes. To this end, we measured whole-body insulin sensitivity using the euglycemic insulin clamp technique in 36 subjects [age 40 ± 9 years, body mass index (BMI) 27.
Human adipocyte differentiation and composition of disease-relevant lipids are regulated by miR-221-3p.
Biochim Biophys Acta Mol Cell Biol Lipids
January 2021
Article Synopsis
- - MicroRNA-221-3p (miR-221-3p) impacts lipid metabolism and adipocyte differentiation, showing that overexpression inhibits fat storage and key adipogenic genes, while its inhibitor promotes fat accumulation.
- - Global lipid analysis revealed that miR-221-3p overexpression disrupts fat synthesis and alters lipid profiles by increasing ceramides and sphingomyelins while decreasing diacylglycerols.
- - Additionally, high levels of miR-221-3p in breast cancer patients' adipose tissue suggest it may enhance cancer cell growth and invasion, indicating a link between fat metabolism and cancer development.