GmHSP40.1, a nuclear-localized soybean J domain protein, participates in regulation of flowering time through interacting with EMF1 and JMJ14.

Heat shock protein 40s (HSP40s) are a group of J domain proteins (JDPs), which serve as co-chaperones for heat shock protein 70s. We previously reported that over-expression of a soybean class C JDP, GmHSP40.1, in Arabidopsis activated defense responses.

Development of a Novel Amplifiable System to Quantify Hydrogen Peroxide in Living Cells.

Although many redox signaling molecules are present at low concentrations, typically ranging from micromolar to submicromolar levels, they often play essential roles in a wide range of biological pathways and disease mechanisms. However, accurately measuring low-abundant analytes has been a significant challenge due to the lack of sensitivity and quantitative capability of existing measurement methods. In this study, we introduced a novel chemically induced amplifiable system for quantifying low-abundance redox signaling molecules in living cells.

Disruption of the Acyl-Activating Enzyme 3 Impairs Seed Coat Mucilage Accumulation and Seed Germination.

The Acyl-activating enzyme () 3 gene encodes an oxalyl-CoA synthetase that catalyzes the conversion of oxalate to oxalyl-CoA as the first step in the CoA-dependent pathway of oxalate catabolism. Although the role of this enzyme in oxalate catabolism has been established, its biological roles in plant growth and development are less understood. As a step toward gaining a better understanding of these biological roles, we report here a characterization of the thaliana () seed mucilage phenotype.

Soybean SAUL1, a Bona Fide U-Box E3 Ligase, Negatively Regulates Immunity Likely through Repressing the Activation of MPK3.

E3 ubiquitin ligases play important roles in plant immunity, but their role in soybean has not been investigated previously. Here, we used (BPMV)-mediated virus-induced gene silencing (VIGS) to investigate the function of SAUL1 (Senescence-Associated E3 Ubiquitin Ligase 1) homologs in soybean. When two closely related homologs were silenced simultaneously, the soybean plants displayed autoimmune phenotypes, which were significantly alleviated by high temperature, suggesting that SAUL1a/1b might be guarded by an R protein.