Phototrophic N and CO Fixation Using a -H Mediated Electrochemical System With Infrared Photons.

A promising approach for the synthesis of high value reduced compounds is to couple bacteria to the cathode of an electrochemical cell, with delivery of electrons from the electrode driving reductive biosynthesis in the bacteria. Such systems have been used to reduce CO to acetate and other C-based compounds. Here, we report an electrosynthetic system that couples a diazotrophic, photoautotrophic bacterium, TIE-1, to the cathode of an electrochemical cell through the mediator H that allows reductive capture of both CO and N with all of the energy coming from the electrode and infrared (IR) photons.

Transcriptome differences in the rumen of beef steers with variation in feed intake and gain.

Background: Feed intake and gain are economically important traits in beef production. The rumen wall interacts with feed, microbial populations, and fermentation products important to cattle nutrition. As such, it is likely to be a critical component in the beef steer's ability to utilize feedstuffs efficiently.

50 years of the Wyoming ram test: How sheep have changed.

Production characteristics of white-faced rams have been systematically evaluated over a 140-d test in Wyoming since 1961. Individual test records ( = 4,240) from rams on test were analyzed to determine change over the past 52 yr. Although rams on test are not older, weight on and off test has increased ( < 0.

Catalytic mechanism of Golgi-resident human tyrosylprotein sulfotransferase-2: a mass spectrometry approach.

Human tyrosylprotein sulfotransferases catalyze the transfer of a sulfuryl moiety from the universal sulfate donor PAPS to the hydroxyl substituent of tyrosine residues in proteins and peptides to yield tyrosine sulfated products and PAP. Tyrosine sulfation occurs in the trans-Golgi network, affecting an estimated 1% of the tyrosine residues in all secreted and membrane-bound proteins in higher order eukaryotes. In this study, an effective LC-MS-based TPST kinetics assay was developed and utilized to measure the kinetic properties of human TPST-2 and investigate its catalytic mechanism when G protein-coupled CC-chemokine receptor 8 (CCR8) peptides were used as acceptor substrates.

Effect of subacute dietary nitrate on production traits and plasma analytes in Suffolk ewes.

Elevated dietary nitrate (NO3-) is associated with production losses in ruminant livestock, resulting in substantial economic losses incurred by producers. Severe drought, fertilization practices and poorly maintained pastures increase the risk of elevated NO3- intake among cattle and sheep. Nitrate is metabolized to nitrite (NO2-) in the rumen and further reduced to ammonia.