Publications by authors named "P A Frisk"
Study Question: Can human pre- and peri-pubertal testicular cells obtained from childhood cancer patients, previously treated with chemotherapy, form testicular organoids (TOs)?
Summary Answer: Organoid formation from testicular tissue collected from childhood cancer patients positively correlates with SRY-Box transcription factor 9 (SOX9) expression in Sertoli cells, which in turn negatively correlates with previous exposure to alkylating chemotherapy.
What Is Known Already: Pre- and peri-pubertal boys exposed to highly gonadotoxic therapies can only safeguard their fertility potential through testicular tissue cryopreservation. Today, there is no established clinical tool to restore fertility using these testicular samples.
Article Synopsis
- The ovarian cortical reserve of follicles is really important for having babies, but some medical treatments can harm them, leading to fertility issues.
- Researchers studied 120 follicles from kids and adults and found two main types of follicles in both age groups, which helps understand their roles better.
- They discovered that while growth patterns are similar, there are some differences in the cells and genes, and they found new marker genes that could improve how we preserve fertility at different ages.
Children with hemato-oncological diseases may have significant skeletal morbidity, not only during and after treatment but also at the time of diagnosis before cancer treatment. This study was designed to evaluate the vitamin D status and circulating bone metabolic markers and their determinants in children at the time of diagnostic evaluation for hemato-oncological disease. This cross-sectional study included 165 children (91 males, median age 6.
View Article and Find Full Text PDFBackground: Gonadotropin-releasing hormone agonists (GnRHa) cotreatment used to transiently suppress ovarian function during chemotherapy to prevent ovarian damage and preserve female fertility is used globally but efficacy is debated. Most clinical studies investigating a beneficial effect of GnRHa cotreatment on ovarian function have been small, retrospective and uncontrolled. Unblinded randomised studies on women with breast cancer have suggested a beneficial effect, but results are mixed with lack of evidence of improvement in markers of ovarian reserve.
View Article and Find Full Text PDFResearch Question: Are age-normalized reference values for human ovarian cortical follicular density adequate for tissue quality control in fertility preservation?
Design: Published quantitative data on the number of follicles in samples without known ovarian pathology were converted into cortical densities to create reference values. Next, a sample cohort of 126 girls (age 1-24 years, mean ± SD 11 ± 6) with cancer, severe haematological disease or Turner syndrome were used to calculate Z-scores for cortical follicular density based on the reference values.
Results: No difference was observed between Z-scores in samples from untreated patients (0.