Photo-toxic effects of 809-nm diode laser and indocyanine green on MDA-MB231 breast cancer cells.

Photodynamic therapy (PDT), due to its positive outcomes in clinical applications, easiness of practice and few side effects, is a good candidate for an efficient treatment of cancer. Indocyanine green (ICG), a water-soluble, anionic tricarbocyanine and non-toxic molecule is a promising photosensitive agent for PDT applications on tumor cells. ICG exhibits strong maximum absorption at around 805 nm which will be an advantage for its use in PDT; light at that wavelength can be used to treat deeper tumors.

Multiple components of photodynamic therapy can phosphorylate Akt.

A growing number of clinically relevant molecular and cellular responses are observed following photodynamic therapy (PDT). PDT-mediated oxidative stress and PDT-induced tissue hypoxia can elicit the transcriptional and/or translational expression of genes associated with cellular stress, inflammation, angiogenesis, immuno-modulation, apoptosis and signal transduction. One of the signaling molecules activated by oxidative stress is Akt/protein kinase B.

Development of a surgical diode-laser system: controlling the mode of operation.

Objective: The aim of this study was to develop a microcontroller based surgical diode laser system and to test it at two different modes (continuous [CW] and modulated) in vitro on lamb liver tissue.

Background Data: In laser surgery, depending on the properties of laser source (wavelength, power, application time, and mode of operation), the effects observed on the tissue may change from carbonization to hyperthermia. The aim is to remove the target tissue without giving any thermal damage to the surrounding tissue.

Closure of skin incisions by 980-nm diode laser welding.

A 980-nm diode laser is proposed to be an alternative welding laser in dermatology due to its optimal penetration in tissue. An in vivo predosimetry study was done to estimate the optimal laser energy delivery conditions (6 W, 400 ms). Next, in vivo experiments were comparatively performed to examine healing of wounds closed either with suture or laser welding.

The biological effects of 632.8-nm low energy He-Ne laser on peripheral blood mononuclear cells in vitro.

The aim of this study was to examine the proliferation of peripheral blood mononuclear cells due to the low energy 632.8-nm He-Ne laser application. The results of previous studies supported the hypothesis that low level laser therapy (LLLT) might have an increasing effect on the proliferation of lymphocytes and production of cytokines.

The 980-nm diode laser for brain surgery: histopathology and recovery period.

The 980-nm diode laser has been under investigation for neurosurgery because of a local peak in the absorption spectra of water around this wavelength. This work was carried out to examine the extent of thermal changes and the recovery process of laser-induced brain lesions. In order to study the quality of the lesions, a conventional monopolar electrocoagulation technique was applied comparatively.