Optimizing decellularization protocols for human thyroid tissues: a step towards tissue engineering and transplantation.

Hypothyroidism is caused by insufficient stimulation or disruption of the thyroid. However, the drawbacks of thyroid transplantation have led to the search for new treatments. Decellularization allows tissue transplants to maintain their biomimetic structures while preserving cell adhesion, proliferation, and differentiation.

Computational drug repurposing for primary hyperparathyroidism.

In hyperparathyroidism (hyperPTH), excessive amounts of PTH are secreted, interfering with calcium regulation in the body. Several drugs can control the disease's side effects, but none of them is an alternative treatment to surgery. Therefore, new drug candidates are necessary.

Combined approaches for detecting polypropylene microplastics in crop plants.

Microplastics (MPs) pollution in the terrestrial environment causes accumulation in crop plants. Consumption of these plants may have negative effects on human health. Therefore, it is crucial to analyze MPs accumulation in the plants.

Production of parathyroid-like cells from thyroid stem cells in co-culture environment.

Background: Parathyroid-like cells were aimed to be developed using cells isolated from thyroid since their embryological origins are the same.

Method: Activin A and sonic hedgehog (Shh) are the proteins used in differentiation (dif) medium. Parathyroid and thyroid cells were cultured in a 3-dimensional environment and divided into five groups: thyroid standard (st) medium, thyroid dif medium, parathyroid st medium, thyroid-parathyroid co-culture st medium, and thyroid-parathyroid co-culture dif medium.

Chondro-inductive hyaluronic acid/chitosan coacervate-based scaffolds for cartilage tissue engineering.

Injuries related to articular cartilage are among the most challenging musculoskeletal problems because of poor repair capacity of this tissue. The lack of efficient treatments for chondral defects has stimulated research on cartilage tissue engineering applications combining porous biocompatible scaffolds with stem cells in the presence of external stimuli. This work presents the role of rat bone marrow mesenchymal stem cell (BMSC) encapsulated-novel three-dimensional (3D) coacervate scaffolds prepared through complex coacervation between different chitosan salts (CHI) and sodium hyaluronate (HA).

Hyaluronic Acid/Chitosan Coacervate-Based Scaffolds.

Chitosan-chloride (CHI) and sodium hyaluronate (HA), two semiflexible biopolymers, self-assemble to form nonstoichiometric coacervates. The effect of counterions was briefly investigated by preparing HA/CHI coacervates in either CaCl or NaCl solutions to find only a small difference in their tendency to coacervate. Higher water content in coacervates within CaCl was attributed to the chaotropic nature of Ca ions.