Perspectives on the Use of Small Noncoding RNAs as a Therapy for Severe Virus-Induced Disease Manifestations and Late Complications.

Many viruses appear each year. Some of these viruses result in severe disease and even death. The frequency of epidemics and pandemics is growing at an alarming rate.

The full recovery of mice (Mus Musculus C57BL/6 strain) from virus-induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs.

Background: Virus-induced cellular genetic modifications result in the development of many human cancers.

Methods: In our experiments, we used the RVP3 cell line, which produce primary mouse virus-induced sarcoma in 100% of cases. Inbreed 4-week-old female C57BL/6 mice were injected subcutaneously in the interscapular region with RVP3 cells.

Reprogramming of CaCo2 colorectal cancer cells after using the complex of poly-(N-vinylpyrrolidone) with small non-coding RNAs.

Small non-coding RNAs control normal development and differentiation in the embryo. These regulatory molecules play a key role in the development of human diseases and are used often today for researching new treatments for different pathologies. In this study, CaCo2 colorectal adenocarcinoma cells were initially epigenetically reprogrammed and transformed into CD4+ cells with nano-sized complexes of amphiphilic poly-(N-vinylpyrrolidone) () with miRNA-152 and piRNA-30074.

Small non-coding RNAs as regulators of structural evolution and carcinogenesis.

Small non-coding RNAs (sncRNAs) are part of non-coding oligonucleotide regulators with wide physiologic and morphologic functions. They control genetic programing of cells, and may modulate processes of differentiation and death. Biogenesis of sncRNAs is now known, and some sncRNAs have been proposed as markers of malignization.

Effects of high tidal volume mechanical ventilation on production of cytokines, iNOS, and MIP-1β proteins in pigs.

The aim of this study was to investigate longitudinal changes of the pulmonary inflammatory process as a result of mechanical stress due to mechanical ventilation. The concentrations of IL-8, TNF-α, MIP-1β, nitrites/nitrates, and inducible nitric oxide synthases (iNOS) were investigated indicate in bronchoalveolar lavage (BAL). Twenty-three piglets were divided into three groups.

Regulation of immune responses, apoptosis, and tumorigenesis by separate FOXP-3-dependent genes: connection with clinical manifestations.

Recently, forkhead/winged-helix family box protein P3 (FOXP-3) was described as the main regulator of regulatory T cells' activity. This transcription factor has the ability to control the immunosuppressive response of regulatory T cells. FOXP-3 has binding sites for different genes specific for proteins with various important functions.

Influence of the lung mechanical ventilation with injurious parameters on 7-ketocholesterol synthesis in Sus Scrofa.

The aim of work was to investigate changes of 7-ketocholesterol synthesis in alveolar macrophages in the dynamic of lung mechanical ventilation with injurious parameters. The goal of in vitro part of work was to observe influence of 7-ketocholesterol on iNOS and MIP1 Beta production in bronchoalveolar lavage fluid (BALF) cells. We used 17 healthy domestic pigs randomly assigned into two treatment groups: group I with mechanical ventilation with physiological parameters; group || underwent injurious ventilation with high volume tidal (VT) and low positive end expiratory pressure (PEEP).