Changes in Membrane Ceramide Pools in Rat Soleus Muscle in Response to Short-Term Disuse.

Lipid raft disruption is an early event during skeletal muscle unloading. Ceramide (Cer) serves as a signaling lipid that can contribute to lipid raft disturbance and muscle atrophy. Using biochemical and fluorescent approaches, the distribution of Cer and related molecules in the rat soleus muscle subjected to 12 h of hindlimb suspension (HS) was studied.

[Sphingolipids in skeletal muscles of C57B1/6 mice after short-term simulated microgravity].

For the first time in skeletal muscle, the sphingolipid profile and key enzymes involved in the generation of ceramide in cells were investigated in simulated microgravity. It was found that, in C57B1/6 mice, the 4-day hindlimb unloading, in addition to reducing the mass of m. soleus, leads to the ceramide accumulation (3-fold) and the decrease of sphingomyelin content in this muscle (7.

[The use of biochips for immunomorphological diagnosis of chronic lymphocytic leukemia].

Immunological biochips (immunobiochips) based on antibodies--test-systems, allowing simultaneously to determine an existence of different antigens in the material. Biochip of this class is usually a plate (substrate), on which antibody molecules with known specificity are immobilized within well-defined areas. The advantage of the use of biochips is the ability to define a large number of antigens at very low flow rates of antibodies.

Immunodiagnosis of chronic lymphocytic leukemia using immunological chips.

The authors present their experience gained in the first clinical use of immunological biochips for detection of cellular surface antigens for immunomorphological diagnosis of chronic lymphocytic leukemia and compare the results of this method and flow cytofluorometry.

[Immunophenotype of neoplastic cells and its clinical significance in chronic lymphocytic leukemia].

y We report preliminary results of efficacious clinical application of a new immunological method with the use of biochips for diagnosis of chronic lymphocytic leukemia. This method has been developed by the authors to detect a wide range of cell surface antigens. We compared the amount of cells expressing certain antigens with clinical properties of chronic lymphocytic leukemia.