Publications by authors named "Otto Gildemeister"
Article Synopsis
- Homologous recombination (HR) is crucial for DNA repair and replication fork progression, primarily involving Rad51 in eukaryotes.
- Recent findings show that Rad51 and related HR proteins like Rad51C and Xrcc3 are present in human mitochondria, highlighting their role in interacting with mitochondrial DNA (mtDNA).
- Depletion of these HR proteins leads to a significant reduction in mtDNA copy numbers and inhibits stress-induced mtDNA replication, indicating their vital function in maintaining the mitochondrial genome.
Article Synopsis
- Cells exposed to DNA-damaging agents show a quick rise in subnuclear complexes with Rad51, suggesting a key role in DNA repair.
- This study found significant increases in nuclear Rad51 levels in HeLa and HCT116 cells after exposure to ionizing radiation.
- Rad51C, which has a nuclear localization signal, appears to facilitate Rad51's transport into the nucleus, as depleting Rad51C led to reduced levels of nuclear Rad51 following DNA damage.
S-Nitrosylation is the modification of a cysteine thiol on a protein or peptide by a nitric oxide (NO) group. Increasing evidence suggests that S-nitrosylation of critical cysteine residues regulates protein function and cell signaling. However, progress in the field has been hampered by a lack of accurate and easy methods for detecting S-nitrosylation and other labile NO-based modifications in samples.
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