Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates.

To improve the quality of in vitro produced (IVP) embryos and reduce pregnancy losses, we proposed to reduce the components of the synthetic oviduct fluid (SOF) medium by 0% (SOF100), 50% (SOF50), and 75% (SOF25). First, embryos produced under these three treatments were evaluated for production, quality, lipid content, gene expression, and methylation patterns. The results indicated that all parameters analyzed were similar across all treatments (P > 0.