A green and sustainable method for monitoring the chemical composition of soybean: an alternative for quality control.

Introduction: Soybean is one of the most important crops in the world, an important source of isoflavones, and used to treat various chronic diseases. High-performance liquid chromatography (HPLC), associated with multivariate experiments and green solvents, is increasingly used to develop comprehensive elution methods for quality control of plants and derivatives.

Objective: The work aims to establish a HPLC fingerprinting method for soybean seeds employing Green Chemistry Principles, a sustainable solvent with low toxicity, and a comprehensive experimental design that reduces the number of experiments.