[Intensity of ultraviolet fluorescence of the cells depending on their size].

Using the light microscope, a correlation between ultra-violet fluorescence intensity of the cell and the cell area was established. This correlation does not depend on the large aperture of the microscope. A possible cause of this correlation is supposed to be either the proportionality between the mass of cytoplasmic protein and the squared cell radius, or a high optical density of cell elements.

[pH-dependence of fluorescence parameters of chymotrypsin, chymotrypsinogen, trypsin and lysozyme].

The alterations of tryptophan fluorescence parametres with pH may be due to: 1) conformational changes; 2) changes in the ionic state of groups capable of quenching the tryptophan fluorescence. The applications of the model of discrete forms of tryptophan allow one to separate these mechanisms and estimate the middle points of conformational changes and pK's of quenching groups. For chymotrypsin (CT) and chymotrypsinogen (CTG) conformational changes were registrated with middle points: CT pH 4.

[Vitamin B6 fluorescence in cells].

The band of cell fluorescence with the maximum of 395-400 nm is registered. This band is exposed on the phone of the tryptophane by wavelength excitation lambdaex=250-260 nm, and in pure scape by lambdaex=310-326 nm. Pyridoxin - substrate of vitamin B6 has identical parameters of spectra excitation and emission of neutral (pH 7) and acid (pH 2) solutions.

[Light scattering by cell suspensions in normal conditions and exposed to external factors].

The characteristics of light scattering of cell suspensions in norm (pH 7,2, t=20degreesC) and upon external influences (change of pH and increase of tdegree). The turbidity tauapproximatelylambda-n and n=0,2--0,3 for cells in norm. After cell damage n increases.