A compact miniaturized continuous flow system for the determination of urea content in milk.

A multicommutation-based flow system with photometric detection was developed, employing an analytical microsystem constructed with low temperature co-fired ceramics (LTCC) technology, a solid-phase reactor containing particles of Canavalia ensiformis DC (urease source) immobilized with glutaraldehyde, and a mini-photometer coupled directly to the microsystem which monolithically integrates a continuous flow cell. The determination of urea in milk was based on the hydrolysis of urea in the solid-phase reactor and the ammonium ions produced were monitored using the Berthelot reaction. The analytical curve was linear in the urea concentration range from 1.

Kinetics independent spectrometric analysis using non-linear calibration modelling and exploitation of concentration gradients generated by a flow-batch system for albumin and total protein determination in blood serum.

An automatic method for kinetics independent spectrometric analysis is proposed in this study. It uses a non-linear calibration model that explores concentration gradients generated by a flow-batch analyser (FBA) for the samples, dye, and the single standard solution. The procedure for obtaining the gradients of the dye and standard solution is performed once at the beginning of analysis.

A flow-injection biamperometric method for determination of pantoprazole in pharmaceutical tablets.

A flow-injection biamperometric method for determination of pantoprazole (PTZ) in pharmaceutical tablets is reported for the first time. The reversible redox couples Fe3+/Fe2+, Fe(CN)6(3-)/Fe(CN)6(4-), Ce4+/Ce3+, VO3(-)NO2+, and I2/I- were tested as indicating redox systems for biamperometric determination of PTZ in a flow-injection assembly with optimized flow parameters. The best results were obtained using V03(-)NO2+, which showed to be a selective and sensitive biamperometric indicating system for PTZ even in the presence of excipients and antioxidants that typically are found in drugs.