Cell labelling and cell damage with indium-111 acetylacetone-an alternative to indium 111 oxine.

The labelling of HeLa S3 cells with 111In acetylacetone (111In-acac) was studied together with cell damage, measured by the reduction in colony-forming ability of labelled cells. Using 2 X 10(5) cells/ml in Hepes saline buffer at pH 7.6 incubated with 7.

Microanalysis of lipids in discrete brain areas of the rabbit following intramuscular administration of steroid contraceptive.

The effects of 60- and 90-day administration of a pill containing 0.05 mg ethinylestradiol and 0.5 mg norgestrel on the regional lipid levels of female albino rabbit brains have been investigated.

Cell damage resulting from the labeling of rat lymphocytes and HeLa S3 cells with In-111 oxine.

Rat thoracic-duct lymphocytes and HeLa S3 cells were labeled in vitro with different amounts of indium-111 oxine. The labeled rat lymphocytes were tested for their ability to recirculate normally in syngeneic rats; the labeled HeLa S3 cells for their ability to divide to form colonies in tissue culture. Both cell types behaved normally by these criteria when labeled with small amounts of indium-111 oxine but at higher doses were obviously damaged.

Stress Metabolites of the Potato and Other Solanaceous Plants.

The effect of stress on the chemical composition of the Solanaceae , primarily Solanum tuberosum (potato), may be profound and have significant health implications. Changes in glycoalkaloids, steroids, sesquiterpenes and other lipids that result from specific and non-specific stress are discussed. The biochemistry and toxicology of these compounds are reviewed.

Disappearance of patulin during alcoholic fermentation of apple juice.

Eight yeast strains were used in three typical American processes to ferment apple juice containing 15 mg of added patulin per liter. Patulin was reduced to less than the minimum detectable level of 50 microgram/liter in all but two cases; in all cases, the level of patulin was reduced by over 99% during alcoholic fermentation. In unfermented samples of apple juice, the concentration of added patulin declined by only 10% when the juice was held for 2 weeks, a period equivalent to the time required for fermentation.

Excretion, distribution and metabolic fate of 3H-alpha-chaconine.

3H-alpha-Chaconine was poorly absorbed from the gastrointestinal tract and rapidly excreted in feces when administered orally to male rats. Intraperitoneal administration of low doses (5 to 10 mg/kg) resulted in urinary and fecal excretion of metabolites, and probably involved biliary excretion. High, toxic, intraperitoneal doses (15 to 25 mg/kg) depressed fecal and urinary elimination, and resulted in accumulation of tritium in various tissues.