Phylogenetic analysis of variants of the Puumala virus (Hantaviridae: ) circulating in the Saratov region.

Unlabelled: The objective is to determine the complete nucleotide sequence and conduct a phylogenetic analysis of genome variants of the Puumala virus isolated in the Saratov region.

Materials And Methods: The samples for the study were field material collected in the Gagarinsky (formerly Saratovsky), Engelssky, Novoburassky and Khvalynsky districts of the Saratov region in the period from 2019 to 2022. To specifically enrich the Puumala virus genome in the samples, were used PCR and developed a specific primer panel.

Determining the Specific Activity of Anti-Rabies Sera and Immunoglobulin Using Atomic Force Microscopy of Cell Cultures.

Background: Mouse neutralization test is widely used to determine the level of anti-rabies antibodies, but it is labor-intensive and time consuming. Alternative methods for determining the neutralizing activity of anti-rabies sera and immunoglobulin in cell cultures are also known. Methods such as FAVN and RFFIT involve the use of fluorescent diagnostics.

A STUDY OF THE ULTRASTRUCTURE OF THE SURFACE OF THE TRANSPLANTABLE LINE VERO CELLS INFECTED WITH THE RABIES VIRUS (RABV, LISSAVIRUS, RHABDOVIRIDAE).

Characteristics of the effect of attenuated rabies virus strain «Moscow 3253» on morphological parameters of transplantable line Vero cells were studied by atomic force microscopy (AFM). Methods based on phase contrast microscopy and immunofluorescence were used to confirm the specificity of interaction and to identify the infectious activity of the rabies virus. Images of intact Vero cells and Vero cells infected with rabies virus were obtained at different periods of cultivation.

[The development of biochip to detect anti-cholera antibodies in human blood serum].

The full-scaled agglutinating immunoassay is commonly applied to detect content of antibodies to cholera agent Vibrio cholerae human in blood serum under application of serological diagnostic. The time of analysis implementation amounts to 18 hours. To shorten time of detection of antibodies a biological microchip (biochip) was developed.

[New approaches to detection of legionellosis causative agents in clinical samples and environment objects by PCR].

Aim: Selection of perspective targets as a base for development of test systems for the detection of legionella DNA in study material by PCR with electrophoresis and hybridization-fluorescent accounting of the results.

Materials And Methods: 22 Legionella pneumophila, 3 Legionella spp. strains and 30 cultures ofheterologic microorganisms, clinical material and environmental object samples were used in the study.

[Development and use of the assay for Legionella pneumophila detection based on fluorescent real-time/endpoint polymerase-chain reaction].

The aim of the work was to develop a PCR-based assay for detection of L. pneumophila and L. micdadei in environmental samples as well as in clinical samples from low respiratory tract and to assess its analytic characteristics.

[The blood serum systems in patients with tuberculosis].

Phenotypes of haptoglobin (Hp), group-specific proteins (Gc), transferrins and ceruloplasmin were studied by means of polyacrylamide gel disk electrophoresis of serum proteins in 411 tuberculosis patients and 283 apparently healthy individuals living in the North-West and Extreme North. Regardless of their ethnic affiliation, tuberculosis patients tend to accumulate Hp 2-2, Gc 1-1 carriers and Hp 2-2 + Gc 1-1 combinations. The persons with the above types of individual proteins usually have a serious clinical picture of the disease and lower activity of the immune T-system, as confirmed by different methods of multidimensional statistical analysis.

[Indices of humoral immunity in inhabitants of the Far North infected and sick with tuberculosis].

As a result of a complex study of the humoral immunity indices in tuberculosis infected and affected inhabitants of the Extreme North with reference to the immunoglobulin A, G, M levels, titers of heterohemagglutinins (HHA) and specific antibodies defined by means of indirect hemagglutination test (IHAT), complement fixation test with tuberculin and IHAT with phosphatidic antigen (IHAT-Ph), two simple and quite informative tests, i.e. HHA and IHAT-Ph, are recommended for the practical use in health system.

[Genetic demographic study of an urban sample of Turkmen-teke with behavioral abnormalities].

Genetic and demographic studies of urban sample of Turkmen-teke (Ashkhabad city) were performed among probands-36 patients with diagnosis of "schizophrenia" and their 840 relatives. Clinical analysis revealed 27.8% of phenocopies.

[Population and demographic study of the Turkmen Tekke].

Genetical and demographic studies of typical rural population of Turkomen teke in Yangi-Kala village of the Ashkhabad region (about 5.000 inhabitants) were performed through the examination of a sample of 3528 persons. The population is characterised by intensive growth (mean number of children per one woman is 6.