Publications by authors named "Oshie A"
A particulate insoluble fraction from Candida albicans NIH B-792 (serotype B) strain cells was obtained as the residue after extracting a 105000 x g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Man alpha 1-->3Man alpha 1-->2Man alpha 1-->Man alpha 1-->2Man, in the presence of GDP-mannose and Mn2+ at pH 6.0 gave a branched mannohexaose, [sequence: see text] 6 the structure of which was identified by means of sequential off assignment.
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- The study isolated a particulate insoluble fraction from Candida albicans J-1012 strain cells using Triton X-100, which led to the analysis of enzyme activity.
- Incubating the fraction with a specific mannopentaose and GDP-mannose resulted in the formation of a new compound, mannohexaose, confirmed by high-performance liquid chromatography and NMR analysis.
- The beta-1,2-mannosyltransferase (ManTase) II activity responsible for this transformation was found to be completely inhibited by Zn2+ and could not be restored by EDTA, showing consistent activity from another Candida strain despite differences in mannan structure.