Bioactive glass-based surfaces induce differential gene expression profiling of osteoblasts.

The ability of Biosilicate® with two crystalline phases (BioS-2P) to drive osteoblast differentiation encourages the investigation of the cellular mechanisms involved in this process. Then, the aim of our study was to analyze the large-scale gene expression of osteoblasts grown on BioS-2P compared with Bioglass 45S5 (45S5). Osteoblasts differentiated from rat bone marrow mesenchymal stem cells were cultured under osteogenic conditions on BioS-2P, 45S5 and polystyrene (control).

Toxocara canis and the allergic process.

The protective effect of infectious agents against allergic reactions has been thoroughly investigated. Current studies have demonstrated the ability of some helminths to modulate the immune response of infected hosts. The objective of the present study was to investigate the relationship between Toxocara canis infection and the development of an allergic response in mice immunised with ovalbumin (OVA).

Evaluation of Crystallized Biosilicate in the Reconstruction of Calvarial Defects.

Introduction: The objective of this study was to assess the bone repair process of crystallized Biosilicate in surgically created defects on rats' calvaria. This biomaterial was recently developed for odontological use.

Materials And Methods: We used fifteen rats (rattus norvegicus albinus, Wistar), and two 5 mm surgical defects were performed on each of them; the defects were made with trephine drill on the calvarium region prior to the biomaterial placement.

A clinical, randomized, controlled study on the use of desensitizing agents during tooth bleaching.

Objectives: To evaluate the efficacy of experimental proposals of desensitizing agents during tooth bleaching.

Methods: 140 participants without tooth sensitivity (TS) received 16% carbamide peroxide (14 days-04 h each) (T1) or 35% hydrogen peroxide (single session-45 min) (T2). Participants used concomitantly (10 per group): desensitizing dentifrices (D1-experimental bioactive glass-ceramic; D2-commercial potassium nitrate; D3-commercial calcium and sodium phosphosilicate) in-home, daily and, desensitizing pastes (D4-experimental bioactive glass-ceramic; D5-experimental Bioglass type 45S5; D6-commercial calcium phosphate), in-office, immediately after the treatment and more 4 times.

Porous bioactive scaffolds: characterization and biological performance in a model of tibial bone defect in rats.

The aim of this study was to evaluate the effects of highly porous Biosilicate(®) scaffolds on bone healing in a tibial bone defect model in rats by means of histological evaluation (histopathological and immunohistochemistry analysis) of the bone callus and the systemic inflammatory response (immunoenzymatic assay). Eighty Wistar rats (12 weeks-old, weighing±300 g) were randomly divided into 2 groups (n=10 per experimental group, per time point): control group and Biosilicate® group (BG). Each group was euthanized 3, 7, 14 and 21 days post-surgery.

Histopathological, cytotoxicity and genotoxicity evaluation of Biosilicate® glass-ceramic scaffolds.

This study evaluated the biocompatibility of Biosilicate® scaffolds by means of histopathological, cytotoxicity, and genotoxicity analysis. The histopathologic analysis of the biomaterial was performed using 65 male rats, distributed into the groups: control and Biosilicate®, evaluated at 7, 15, 30, 45, and 60 days after implantation. The cytotoxicity analysis was performed by the methyl thiazolyl tetrazolium (MTT) assay, with various concentrations of extracts from the biomaterial in culture of osteoblasts and fibroblasts after 24, 72, and 120 h.

Effects of biosilicate and bioglass 45S5 on tibial bone consolidation on rats: a biomechanical and a histological study.

The purpose of this study was to investigate the effects of Bioglass 45S5 and Biosilicate, on bone defects inflicted on the tibia of rats. Fifty male Wistar rats were used in this study, and divided into five groups, including a control group, to test Biosilicate and Bioglass materials of two different particle sizes (180-212 microm or 300-355 microm). All animals were sacrificed 15 days after surgery.

Evaluation of the thermal shrinkage of titanium and the setting and thermal expansion of phosphate-bonded investments.

Statement Of Problem: There are few studies on titanium casting shrinkage, and phosphate-bonded investments for titanium casting have not produced appropriate marginal fit.

Purpose: The purpose of this study was to determine the thermal shrinkage of titanium and the setting and thermal expansion of 3 phosphate-bonded investments.

Material And Methods: The thermal shrinkage between the melting temperature and room temperature was calculated using a titanium thermal expansion coefficient.