Publications by authors named "Oscar Juez"
PLoS Comput Biol
March 2021
Article Synopsis
- The text introduces a new method called pAC-Seq, which allows scientists to observe guide RNAs directly in single-cell RNA sequencing (scRNA-seq).
- pAC-Seq is used to study the effects of CRISPR/Cas9 gene edits on how genes are regulated and expressed, specifically focusing on alterations to gene cis-regulatory regions.
- The method can detect changes in gene expression due to regulatory alterations even when only a small percentage (about 5%) of cells show complete gene loss, highlighting the need for sufficient cell sampling to observe these effects, which can be improved by targeted sequencing of transcripts.
