Outcomes and DNA analysis of ex vivo expanded stem cell allograft for ocular surface reconstruction.

Purpose: To investigate the outcome of a new technique of ex vivo expanded stem cell allograft for limbal stem cell deficiency (LSCD), and to characterize the ocular surface genotype after surgery.

Design: Retrospective noncomparative case series.

Participants: Ten eyes of 10 patients with profound LSCD arising from ectodermal dysplasia (3 eyes), Stevens-Johnson syndrome (3 eyes), chemical injury (2 eyes), thermal injury (1 eye), and rosacea blepharoconjunctivitis (1 eye).

Poliosis associated with treatment of fungal endophthalmitis.

Purpose: To report a case of poliosis arising from treatment with topical medications.

Design: Interventional case report.

Methods: A 37-year-old man was treated for fungal endophthalmitis arising from Aspergillus fumigatus keratitis with penetrating keratoplasty, lensectomy and vitrectomy together with systemic and prolonged topical medications.

Reproducibility of LASIK flap thickness using the Hansatome microkeratome.

Purpose: To evaluate the actual versus the expected thickness of laser in situ keratomileusis (LASIK) flaps and to determine the factors that affect flap thickness.

Setting: Centre For Sight, Queen Victoria Hospital, East Grinstead, United Kingdom.

Methods: A retrospective analysis of LASIK procedures in 757 consecutive eyes was done.

Unexpected flap thickness in laser in situ keratomileusis.

We report a case of an unexpected thick flap during laser in situ keratomileusis (LASIK) that led to abandonment of surgery. This report illustrates the importance of stromal bed measurements after flap creation in LASIK. A thicker-than-expected flap can lead to a thinner-than-anticipated residual cornea and subsequent ectasia or even perforation during laser ablation.