Monitoring of estrous cycle of ewes by ram-seeking behavior.

For 6 years adult, non-synchronized Hampshire ewes (n=2,5,8,8,9 and 12) and rams (n=6,10,12,11,11 and 9) were maintained separately and released to contiguous pastures having in common a woven-wire fence about 80m long and 1.1m high. Natural estrous cycles were monitored daily by observations of ram-seeking behavior as defined as a ewe being persistent in staying at the common fence with attention focused on the ram to the virtual exclusion of grazing.

BUB/BnJ (H-2q) is a TCR deletion mutant mouse strain (TCR V beta a, KJ16-) that is susceptible to type II collagen-induced arthritis.

Type II collagen-induced arthritis (CIA) in mice is an animal model of autoimmune inflammatory arthritis. Arthritis is induced in susceptible strains of mice (H-2q and H-2r) after immunization with heterologous or autologous type II collagen in CFA. Induction of CIA is T cell dependent and a restricted usage of TCR V beta genes has been found in the arthritic joints and lymph nodes of mice with CIA.

Immunocytochemical Localization of Glia-Derived Nexin, Laminin and Fibronectin on the Surface or Extracellular Matrix of C6 Rat Glioma Cells, Astrocytes and Fibroblasts.

The expression and cellular distribution of glia-derived nexin (GDN), laminin and fibronectin on C6 rat glioma cells, rat brain astrocytes and rat fibroblasts were investigated by immunoblotting and immunocytochemistry. Western blot analysis of C6 cell homogenates confirmed the specificities of the antibodies. Immunocytochemical staining of C6 cells, astrocytes and fibroblasts showed that laminin, fibronectin and GDN were abundant on the surface of glioma cells and astrocytes whereas on fibroblasts fibronectin was abundant though only traces of GDN and laminin could be detected.

The effect of age, pairing, copulation, and 2-Br-alpha-ergocryptine in the male Mongolian gerbil on the prolactin cells in the anterior pituitary identified by immunocytochemical and Herlant's tetrachrome methods.

Near-adjacent, midsagittal sections of pituitaries of infantile and adult male Mongolian gerbils in several experimental groups (bachelor, paired, tartaric acid-injected, postcopulatory gerbil, and postcopulatory animal injected with 2-bromo-alpha-ergocryptine [CB-154]), were stained by a modification of Herlant's tetrachrome and by the peroxidase-labeled antibody method for prolactin. Cell counts of erythrosinophils and cells immunoreactive for prolactin were made. Sequential staining procedures and thin adjacent sections were used to correlate the staining results.