Cellular Attachment Assays to Dissect Molecular Drivers of Epidermal Cell Function.

Cell attachment is the process by which cells interact with their environment, including neighboring cells and the extracellular matrix (ECM). Attachment plays a critical role in maintaining skin integrity, promoting wound healing, and facilitating cellular communication in epidermal cells, such as keratinocytes. However, the many different factors that can influence this mechanism make it challenging to recapitulate in cellular models.

Computation-guided scaffold exploration of 2,6-1,10-eunicellanes.

Eunicellane diterpenoids are a unique family of natural products containing a foundational 6/10-bicyclic framework and can be divided into two main classes, and , based on the configurations of their ring fusion at C1 and C10. Previous studies on two bacterial diterpene synthases, Bnd4 and AlbS, revealed that these enzymes form - and -eunicellane skeletons, respectively. Although the structures of these diterpenes only differed in their configuration at a single position, C1, they displayed distinct chemical and thermal reactivities.

Antioxidant Response, Phenolic Compounds and Yield of Tubers Inoculated with Arbuscular Mycorrhizal Fungi and Growing under Water Stress.

(potato) is one of the most common crops worldwide; however, it is sensitive to water stress, which necessitates the identification of alternative tools to improve their production. Here, we evaluated the inoculation of two arbuscular mycorrhizal fungi (AMF) strains, (CC), (HMC26), and the MIX (CC + HMC26) in yield and phenolic and antioxidant response using chromatographic and spectroscopic methods in potato crops, at increasing levels of water stress, namely, with 100% (0), 70% (S1), and 40% (S2) soil humidity. Two caffeoylquinic acid isomers were detected and their levels showed a tendency to increase under stress together with the AMF inoculation, reaching up to 19.

Correlating electrochemical stimulus to structural change in liquid electron microscopy videos using the structural dissimilarity metric.

In-situ liquid cell transmission electron microscopy (LCTEM) with electrical biasing capabilities has emerged as an invaluable tool for directly imaging electrode processes with high temporal and spatial resolution. However, accurately quantifying structural changes that occur on the electrode and subsequently correlating them to the applied stimulus remains challenging. Here, we present structural dissimilarity (DSSIM) analysis as segmentation-free video processing algorithm for locally detecting and quantifying structural change occurring in LCTEM videos.

Distribution, reactivity and vertical fluxes of methane in the Guadalquivir Estuary (SW Spain).

The influence of temperature, salinity, sediment-water-atmosphere exchanges and oxidation rate on the variability of methane (CH) in the Guadalquivir Estuary has been studied. The database corresponds to 3 intensive samplings carried out in summer (2021 and 2022) and winter (2022). An increase in CH concentration towards the interior of the estuary has been observed, more intense during summer (19-371 nmol L).

Dynamics of CO, CH, and NO in Ria Formosa coastal lagoon (southwestern Iberia) and export to the Gulf of Cadiz.

A first characterization of greenhouse gases had been carried out to study their role and impact in a productive transitional coastal system of the southern Portugal - Ria Formosa lagoon. To this purpose, the partial pressure of CO (pCO) and the concentration of dissolved CH and NO have been measured. Two surveys were carried out during 2020, at low tide under typical conditions of Spring (March) and end of Summer (October).

A numerical simulation study of the hydrodynamic effects caused by morphological changes in the Guadalquivir River Estuary.

This study presents an analysis of the impacts of the changes in bottom depth along the Guadalquivir Estuary on tidal dynamics. A realistic non-linear 1D numerical model, incorporating changes in both breadth and bottom depth, was employed to investigate the involved effects. The findings reveal a significant amplification of the M2 tidal wave towards the upper region of the Estuary, resulting from the gradual deepening caused by multiple dredging operations.

Greenhouse gas assemblages (CO, CH and NO) in the continental shelf of the Gulf of Cadiz (SW Iberian Peninsula).

This study examines the simultaneous water-atmosphere exchange of carbon dioxide (CO), methane (CH) and nitrous oxide (NO) on the continental shelf of the Gulf of Cadiz, as well as the effect it has in terms of the radiative balance in the atmosphere, between 2014 and 2016. The experimental database consists of new measurements of the spatial and seasonal distribution of CO partial pressure (pCO) and NO concentration in 2016. pCO shows a wide range of variation influenced mainly by seasonal thermal variations (8.

Dissolved organic matter distribution in the water column and sediment pore water in a highly anthropized coastal lagoon (Mar Menor, Spain): Characteristics, sources, and benthic fluxes.

Coastal lagoons are among the most productive and biodiverse systems in the world and are important sentinels of climate change. The Mar Menor is one of the largest coastal lagoons in the Mediterranean, providing a variety of ecosystem services and resources to the community. However, in recent decades this lagoon has suffered drastic changes and degradation caused by human activities.

First -eunicellane terpene synthase in bacteria.

Terpenoids are the largest family of natural products, but prokaryotes are vastly underrepresented in this chemical space. However, genomic supports vast untapped biosynthetic potential for terpenoids in bacteria. We discovered the first -eunicellane terpene synthase (TS), AlbS from NRRL B-1670, in nature.

Phytotherapeutic alternatives for neurodegenerative dementias: Scientific review, discussion and therapeutic proposal.

The incidence and prevalence of age-related neurodegenerative dementias have been increasing. There is no curative therapy and conventional drug treatment can cause problems for patients. Medicinal plants traditionally used for problems associated with ageing are emerging as a therapeutic resource.

Impact of the Nutritional Status on Clinical Outcomes of Patients with Coronavirus Disease Undergoing Cancer Treatment.

Coronavirus disease (COVID-19) is a critical and potentially fatal condition. The nutritional status affects the evolution and clinical outcome throughout the disease course among factors influencing the patient prognosis. In patients with cancer, the Patient-Generated Subjective Global Assessment (PG-SGA) is the preferred instrument for assessing the nutritional status.

Investigation of Acid-Base Catalysis in Halimadienyl Diphosphate Synthase Involved in Virulence.

The devastating human pathogen (Mtb) is able to parasitize phagosomal compartments within alveolar macrophage cells due, in part, to the activity of its cell-surface lipids. Prominent among these is 1-tuberculosinyl-adenosine (1-TbAd), a derivative of the diterpenoid tuberculosinyl (halima-5,13-dienyl) diphosphate produced by the class II diterpene cyclase encoded by Rv3377c, termed here MtHPS. Given the demonstrated ability of 1-TbAd to act as a virulence factor for Mtb and the necessity for Rv3377c for its production, there is significant interest in MtHPS activity.

Bighorn sheep show similar in-host responses to the same pathogen strain in two contrasting environments.

Ecological context-the biotic and abiotic environment, along with its influence on population mixing dynamics and individual susceptibility-is thought to have major bearing on epidemic outcomes. However, direct comparisons of wildlife disease events in contrasting ecological contexts are often confounded by concurrent differences in host genetics, exposure histories, or pathogen strains. Here, we compare disease dynamics of a spillover event that affected bighorn sheep populations in two contrasting ecological contexts.

Description of collaborative, fee-for-service, office-based, pharmacist-directed medical cannabis therapy management service for patients with chronic pain.

Background: Treatment with medical cannabis (MC) in the United States tends to be patient-driven in nature despite evidence that suggests that patients have remarkably poor knowledge on the medical use of this treatment modality.

Objective: To develop and pilot a collaborative, fee-for-service (FFS), office-based, pharmacist-directed MC therapy management (MCTM) service for patients suffering chronic pain.

Practice Description: A collaborative, FFS, office-based, pharmacist-directed MCTM service where patients are seen after a physician deems them suitable for treatment with MC.

Dynamic of CO, CH and NO in the Guadalquivir estuary.

The concentration of dissolved CH and NO, as well as the partial pressure of CO (pCO) were studied in the Guadalquivir estuary. Samples were taken in March and April 2018 and 2019, under different rainy and tidal conditions. The available database for summer 2017 (Sierra et al.

Linkages between greenhouse gases (CO CH and NO) and dissolved organic matter composition in a shallow estuary.

Estuarine systems receive large amounts of organic matter that enhance the production of greenhouse gases (GHGs), such as carbon dioxide (CO), methane (CH), and nitrous oxide (NO). Despite considerable research on GHGs and dissolved organic matter (DOM) distribution in estuaries, little is known about the linkage between these gases and DOM composition. Here we evaluated the relationship between three GHGs (CO, CH, and NO) and DOM composition, determined through optical properties, in Guadalete estuary (Bay of Cadiz, Spain).

"Greenhouse gas dynamics in a coastal lagoon during the recovery of the macrophyte meadow (Mar Menor, SE Spain)".

The Mar Menor is a hypersaline coastal lagoon with salinity values ranging from 41.9 to 45.5.

Medicinal plants and natural products as neuroprotective agents in age-related macular degeneration.

The retina may suffer neurodegenerative damages, as other tissues of the central nervous system do, and serious eye diseases may develop. One of them is age-related macular degeneration, which causes progressive loss of vision due to retina degeneration. Treatment of age-related macular degeneration focuses on antioxidant agents and anti-vascular endothelial growth factor compounds, among others, that prevent/diminish oxidative stress and reduce neovascularisation respectively.

Singlet oxygenation of triquinacene, barrelene, and homobarrelene.

The singlet oxygenation of three polycyclic hydrocarbons, triquinacene, barrelene and homobarrelene was studied. Triquinacene reacted by way of a perepoxide intermediate, transferring an oxygen atom to another triquinacene molecule to give exclusively the mono epoxide. Barrelene, on the other hand, underwent a rare homo-Diels-Alder reaction with O to give the decomposition product from the initial tetracyclic 1,2-dioxolane leading to benzofuran.

Neuroprotective Potential of Ginkgo biloba in Retinal Diseases.

Like other tissues of the central nervous system, the retina is susceptible to damage by oxidative processes that result in several neurodegenerative disease such as age-related macular degeneration, diabetic retinopathy, glaucoma, ischaemic retinal disease, retinal disease produced by light oxidation, and detached retina, among other diseases. The use of antioxidant substances is a solution to some health problems caused by oxidative stress, because they regulate redox homeostasis and reduce oxidative stress. This is important for neurodegeneration linked to oxidation processes.

Improving university students' web savvy: An intervention study.

Background: Young people increasingly turn to the Internet for information about social and political issues. However, they struggle to evaluate the trustworthiness of the information they encounter online.

Aims: This pilot study investigated whether a focused curricular intervention could improve university students' ability to make sound judgements of credibility.

Preliminary evaluation of the efficacy, safety, and costs associated with the treatment of chronic pain with medical cannabis.

Introduction: Medical cannabis (MC) is commonly claimed to be an effective treatment for chronic or refractory pain. With interest in MC in the United States growing, as evidenced by the 29 states and 3 US districts that now have public MC programs, the need for clinical evidence supporting this claim has never been greater.

Methods: This was a retrospective, mirror-image study that investigated MC's effectiveness in patients suffering from chronic pain associated with qualifying conditions for MC in New York State.

Temporal and spatial variation of NO production from estuarine and marine shallow systems of Cadiz Bay (SW, Spain).

There is still much uncertainty regarding the global oceanic emissions of NO, and particularly emissions from coastal regions, because spatio-temporal datasets have limited coverage. The concentration of dissolved NO in surface waters and the associated fluxes to the atmosphere have been studied in three coastal systems located near Cadiz Bay (southwestern coast of Spain) over different time scales. The three systems present different hydrodynamic characteristics (an estuary and two marine systems) that influence the distribution of NO in the water column.