[The biological and diagnostic implication of the comparative data on expression of the same protein, obtained by Western blotting and surface plasmone resonance tests].

The comparative analysis of the data on the expression of the same modulating protein MX, obtained on the same biological materials by Western blotting and surface plasmone resonance tests was shown to provide in most cases a semiquantitative opinion as to modulation of MX affinity to its specific target (effector X protein) under the experimental action of F. This is doubtlessly to offer additional possibilities for the prediction and differential diagnosis of a number of diseases, including cancer.

[Characteristics of polyamine biosynthesis regulation and tumor growth rate in hormone-dependant grafted breast tumors of mice and rats].

Effect of the inhibitors of polyamines biosynthesis on completely or partially hormone-dependant breast tumors (mouse Ca755 carcinoma and Walker W-256 carcinosarcoma) is essentially special: in contrary to hormone-dependant tumors, this effect may be not only breaking but stimulating as well. Change-over from one to another mode of reaction is conditioned, most probable, by hormonal status, which is determined by one or another estral cycle phase. Biochemical mechanisms of this change-over are closely connected with polyamines metabolism, namely the degree of polyamines (especially spermine) interconvertion and physiological reactivity level of the system controlling expression of ornithin-decarboxilase.

[A comparative study of the asymmetry in the spatial-exploratory behavior of mice in 2 populations].

In two populations of contemporary generations of non-strain mice was found a considerable shift of interhemispheric asymmetry the relative activity of the right hemisphere being increased in comparison with the known data. Quantitative evaluation and the character of this shift in the two populations were different. Changes observed are suggested to be a manifestation of adaptive response of the organism to environmental pollution.

[The effect of peripheral cholinergic blockers on the cocarcinogenic action of an experimental influenza infection].

The cocarcinogenic effect of chronic influenza infection was shown to be mediated by M-cholinergic structures. Administration of a M-anticholinergic agent in low dose at the key stages of chronic influenza infection development was followed by a nearly complete suppression of its cocarcinogenic action. N-anticholinergic agents failed to exert such effect.

[The biological effects in animals in relation to the accident at the Chernobyl Atomic Electric Power Station. 10. Cooperative immune reactions in different generations of mice].

The immune status of mice has been assessed by the whole complex of data. The permanent action of low-level radiation has been shown to suppress considerably the rate of reactions of the delayed-type hypersensitivity and "graft versus host" disease, as well as NK and specific cytolytic T-lymphocyte activity. The dynamics of accumulation and the levels of antiviral antibodies in the serum, lung and trachea extracts are virtually invariable.

[The immunocorrective effect of laser reflexotherapy in experimental influenza infection].

The influence of two schemes of laser acupuncture on some cell-mediated and humoral immunity characteristics of mice, as well as on their nonspecific antiviral resistance, in acute experimental influenza infection has been studied. The use of both schemes has been found to considerably decrease the severity of infection, enhancing the activity of lymphocytes of infected mice in the graft versus host reaction, the O2-producing activity of alveolar macrophages and modulating the ratio of antihemagglutinins and nonspecific antiviral inhibitors in the blood serum.

[The effect of a superhigh dose of ionizing radiation on the content of catecholamine mediators in different areas of the rat brain].

In experiments with rats, it was shown that whole-body irradiation of animals with a dose of 500 Gy causes a decrease in the catecholamine mediator content of the truncus cerebri during the first minutes following irradiation. A possible mechanism of the changes observed is discussed.

[Secondary intensification of influenza virus reproduction and the development of tumors in mice].

Tumor disposition in mice with chronic influenza infection was shown to be completed chiefly during secondary increase in viral reproduction. This condition presents with various systemic disorders and a pronounced increase in susceptibility to the cocarcinogenic effect of polymetal aerosols.

[Interaction of amino acyl-tRNA-synthetases from the rabbit liver with RNA and polyanions].

The interaction of aminoacyl-tRNA synthetase with RNA and polyanions was studied. The inhibition of the enzymes by polyU, polyI and heparin was demonstrated. It was found that this interaction is of limited specificity and is typical of single-stranded RNAs which possess no orderly secondary structure as well as of other polyanions possessing similar polyelectrolytic properties.

[Isolation, polypeptide composition and properties of aminoacyl-tRNA-synthetase complexes from the rabbit liver].

The procedure for isolation of the aminoacyl-tRNA-synthetase complex from rabbit liver based on the affinity chromatography on heparin- and tRNA-Sepharose has been developed. The complex has a Mr of about 1100 kD and is made up of 10 polypeptides, eight of which are aminoacyl-tRNA synthetase. The complex stability was studied under various conditions.

[Correlation of the hypoxic effects in the bone marrow and subcutaneous fatty tissue of rats at different exposures].

In acute experiments on rats different agents and their combinations were used to induced hypoxia. In order to estimate the oxygen status of crus bone marrow by taking measurements in subcutaneous fat by a standard electrode with small working surface several electrodes should be used simultaneously. A correlation between subcutaneous and bone-marrow hypoxic tension caused by the hypoxia-induced agents was associated with the subcutaneous fat zone under study.

[Possible role of the macromolecular environment in enzyme functioning in the cell. Interaction of E. coli beta-galactosidase with endogenous polycationic proteins and kinetic parameters of the enzyme in situ].

The effect of endogenous protein polycations on the kinetic properties of beta-galactosidase was studied. The dependence of kinetic properties of the enzyme (Km and V) in situ at the growth stage of microbial cultures was demonstrated. The observed phenomenon may be explained by the enzyme interaction with endogenous polycations.

[Development of lung tumors in BALB/c mice in chronic influenza infection and inhalation of metal-containing aerosols].

Intensification of cocarcinogenic effect of chronic influenza infection (10 months after virus instillation) in BALB/c mice was obtained by polymetallic aerosol inhalation 5 or 6 months after infection. An increase of malignant neoplasms incidence was accompanied by a decrease of the cytoproliferative activity in the thymus and spleen. The immunopathogenic and cocarcinogenic effects were maximal when the aerosol treatment was carried out 6 months after infection.

[Normalizing effect of interferon on the system of mixed-function oxidases in the liver of mice with Lewis lung carcinoma].

The effect of interferon (IF) on the activity of multipurpose hepatic oxidases (MHO) in animals in the process of metastatic tumour development as well as interaction of IF and vinblastine (VBL) in the cell culture have been studied. IF activates MHO under conditions of their inhibition at the beginning of the tumour development and inhibits them to the normal level under activation in the postmetastatic period. Contrary to the data obtained in vivo IF does not decrease the toxicity of VBL for the tumour cells in vitro.

[Isolation and properties of prostaglandin H synthetase immobilized in insoluble polyelectrolyte complexes].

Immobilization of prostaglandin-H-synthetase (EC 1.14.99.

[Formation of lactate dehydrogenase complexes with proteins and polyelectrolytes. A possible physiological role].

The effect of polymers (proteins, polyaminoacids, polyethylenimine) on kinetic parameters of lactate dehydrogenase (LDH) from porcine skeletal muscle was studied. Activation of the enzyme which was partially due to the association of LDH dimers was observed. A hypothesis was proposed, according to which the contribution of dissociation of oligomeric enzymes in the regulation of their activity in vivo is negligible due to the equilibrium shift towards association in dissociable enzyme systems.

[The role of the hypoxic mechanism in the radioprotective effect of mezaton].

The method of coupled estimation of the hypoxic and radioprotective effect has demonstrated that the hypoxic mechanism does not play an essential role in the radioprotective action of phenylephrine hydrochloride.