Publications by authors named "Orie Terai"
Article Synopsis
- - The study focused on the safety concerns of residual undifferentiated pluripotent stem cells (PSCs) in cell therapy products (CTPs), which could lead to tumor formation in clinical applications.
- - Researchers tested a droplet digital PCR (ddPCR) method to detect leftover induced pluripotent stem cells (iPSCs) in cardiomyocyte samples by varying their concentrations and measuring specific iPSC markers for accuracy.
- - Findings indicated that the RT-ddPCR assay effectively identifies iPSC impurities in CTPs, with variability primarily arising from how iPSCs were added to samples, showcasing the method's potential for ensuring safety in PSC-derived therapies.
Background Aims: The administration of human cell-processed therapeutic products (hCTPs) is associated with a risk of tumorigenesis due to the transformed cellular contaminants. To mitigate this risk, these impurities should be detected using sensitive and validated assays. The digital soft agar colony formation (D-SAC) assay is an ultrasensitive in vitro test for detecting tumorigenic transformed cells in hCTPs.
View Article and Find Full Text PDFThree-dimensional retinal organoids (3D-retinas) are a promising graft source for transplantation therapy. We previously developed self-organizing culture for 3D-retina generation from human pluripotent stem cells (hPSCs). Here we present a quality control method and preclinical studies for tissue-sheet transplantation.
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