Publications by authors named "Oriane Duchamp de Lageneste"
Article Synopsis
- Congenital pseudarthrosis of the tibia (CPT) is characterized by non-healing bone fractures, often linked to neurofibromatosis type 1 (NF1), a genetic disorder caused by mutations in a tumor suppressor gene.
- Research focused on the role of specific cell types, including Schwann cells and skeletal stem/progenitor cells (SSPCs), in the development of fibrosis associated with CPT.
- Treatment using inhibitors targeting the RAS-MAPK signaling pathway showed promise in preventing fibrous nonunion in a mouse model, suggesting a new potential strategy for dealing with CPT-related complications.
The periosteum covering the outer surface of bone contains skeletal stem/progenitor cells that can efficiently form cartilage and bone during bone repair. Several methods have been described to isolate periosteal cells based on bone scraping and/or enzymatic digestion. Here, we describe an explant culture method to isolate periosteum-derived stem/progenitor cells for subsequent and analyses.
View Article and Find Full Text PDFMost organs and tissues in the body, including bone, can repair after an injury due to the activation of endogenous adult stem/progenitor cells to replace the damaged tissue. Inherent dysfunctions of the endogenous stem/progenitor cells in skeletal repair disorders are still poorly understood. Here, we report that Fgfr3 over-activating mutation in Prx1-derived skeletal stem/progenitor cells leads to failure of fracture consolidation.
View Article and Find Full Text PDFBone regeneration is an efficient regenerative process depending on the recruitment and activation of skeletal stem cells that allow cartilage and bone formation leading to fracture consolidation. Periosteum, the tissue located at the outer surface of bone is now recognized as an essential player in the bone repair process and contains skeletal stem cells with high regenerative potential. The matrix composition of the periosteum defines its roles in bone growth, in cortical bone modeling and remodeling in response to mechanical strain, and in bone repair.
View Article and Find Full Text PDFBone regeneration relies on the activation of skeletal stem cells (SSCs) that still remain poorly characterized. Here, we show that periosteum contains SSCs with high bone regenerative potential compared to bone marrow stromal cells/skeletal stem cells (BMSCs) in mice. Although periosteal cells (PCs) and BMSCs are derived from a common embryonic mesenchymal lineage, postnatally PCs exhibit greater clonogenicity, growth and differentiation capacity than BMSCs.
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