Publications by authors named "Ori Heyman"
Encounters between host cells and intracellular bacterial pathogens lead to complex phenotypes that determine the outcome of infection. Single-cell RNA sequencing (scRNA-seq) is increasingly used to study the host factors underlying diverse cellular phenotypes but has limited capacity to analyze the role of bacterial factors. Here, we developed scPAIR-seq, a single-cell approach to analyze infection with a pooled library of multiplex-tagged, barcoded bacterial mutants.
View Article and Find Full Text PDFProlonged metabolic stress can lead to severe pathologies. In metabolically challenged primary fibroblasts, we assigned a novel role for the poorly characterized miR-4734 in restricting ATF4 and IRE1-mediated upregulation of a set of proinflammatory cytokines and endoplasmic reticulum stress-associated genes. Conversely, inhibition of this miRNA augmented the expression of those genes.
View Article and Find Full Text PDFArticle Synopsis
- Mutations similar to those caused by growth factors lead to increased proliferation and movement in aggressive types of breast tumors, prompting the need to explore new players in this process.
- Researchers conducted a phosphoproteomic analysis on normal mammary cells treated with epidermal growth factor (EGF) and identified a protein called ladinin-1 (LAD1) that plays a critical role in mediating responses in the EGF-to-ERK pathway.
- Further studies showed that LAD1 is involved in cell proliferation and migration, interacts with actin and related proteins, and its depletion negatively impacts cell survival, making it a potential predictor of poor prognosis in aggressive breast cancer types like triple-negative and HER2-positive tumors.