Dietary Methionine and Total Sulfur Amino Acid Restriction in Healthy Adults.

Objectives: Dietary restriction of methionine (Met) and cysteine (Cys) delays the aging process and aging-related diseases, improves glucose and fat metabolism and reduces oxidative stress in numerous laboratory animal models. Little is known regarding the effects of sulfur amino acid restriction in humans. Thus, our objectives were to determine the impact of feeding diets restricted in Met alone (MetR) or in both Met and Cys (total sulfur amino acids, SAAR) to healthy adults on relevant biomarkers of cardiometabolic disease risk.

Cumulative Consumption of Sulfur Amino Acids and Risk of Diabetes: A Prospective Cohort Study.

Background: Cross-sectional studies have suggested that consumption of sulfur amino acids (SAAs), including methionine and cysteine, is associated with a higher risk of type 2 diabetes (T2D) in humans and with T2D-related biomarkers in animals. But whether higher long-term SAA intake increases the risk of T2D in humans remains unknown.

Objectives: We aimed to investigate the association between long-term dietary SAA intake and risk of T2D.

Methionine-restricted diet inhibits growth of MCF10AT1-derived mammary tumors by increasing cell cycle inhibitors in athymic nude mice.

Background: Dietary methionine restriction (MR) improves healthspan in part by reducing adiposity and by increasing insulin sensitivity in rodent models. The purpose of this study was to determine whether MR inhibits tumor progression in breast cancer xenograft model and breast cancer cell lines.

Methods: Athymic nude mice were injected with MCF10AT1 cells in Matrigel® and fed a diet containing either 0.

Dietary methionine restriction in mice elicits an adaptive cardiovascular response to hyperhomocysteinemia.

Dietary methionine restriction (MR) in rodents increased lifespan despite higher heart-to-body weight ratio (w/w) and hyperhomocysteinemia, which are symptoms associated with increased risk for cardiovascular disease. We investigated this paradoxical effect of MR on cardiac function using young, old, and apolipoprotein E-deficient (ApoE-KO) mice. Indeed, MR animals exhibited higher heart-to-body weight ratio (w/w) and hyperhomocysteinemia with a molecular pattern consistent with cardiac stress while maintaining the integrity of cardiac structure.

The first international mini-symposium on methionine restriction and lifespan.

It has been 20 years since the Orentreich Foundation for the Advancement of Science, under the leadership Dr. Norman Orentreich, first reported that low methionine (Met) ingestion by rats extends lifespan (Orentreich et al., 1993).

Methionine restriction prevents the progression of hepatic steatosis in leptin-deficient obese mice.

Objective: This study investigated the effects of dietary methionine restriction (MR) on the progression of established hepatic steatosis in the leptin-deficient ob/ob mouse.

Material/methods: Ten-week-old ob/ob mice were fed diets containing 0.86% (control-fed; CF) or 0.

Methionine-restricted C57BL/6J mice are resistant to diet-induced obesity and insulin resistance but have low bone density.

Dietary methionine restriction (MR) extends lifespan, an effect associated with reduction of body weight gain, and improvement of insulin sensitivity in mice and rats as a result of metabolic adaptations in liver, adipose tissue and skeletal muscle. To test whether MR confers resistance to adiposity and insulin resistance, C57BL/6J mice were fed a high fat diet (HFD) containing either 0.86% methionine (control fed; CF) or 0.

Effect of taurine and N-acetylcysteine on methionine restriction-mediated adiposity resistance.

Objectives: Methionine-restricted (MR) rats, which are lean and insulin sensitive, have low serum total cysteine (tCys) and taurine and decreased hepatic expression and activity indices of stearoyl-coenzyme A desaturase-1 (SCD1). These effects are partly or completely reversed by cysteine supplementation. We investigated whether reversal of MR phenotypes can be achieved by other sulfur compounds, namely taurine or N-acetylcysteine (NAC).

Genomic and metabolic responses to methionine-restricted and methionine-restricted, cysteine-supplemented diets in Fischer 344 rat inguinal adipose tissue, liver and quadriceps muscle.

Background/aims: Methionine restriction (MR) is a dietary intervention that increases lifespan, reduces adiposity and improves insulin sensitivity. These effects are reversed by supplementation of the MR diet with cysteine (MRC). Genomic and metabolomic studies were conducted to identify potential mechanisms by which MR induces favorable metabolic effects, and that are reversed by cysteine supplementation.

Metabolic adaptations to methionine restriction that benefit health and lifespan in rodents.

Restriction of dietary methionine by 80% slows the progression of aged-related diseases and prolongs lifespan in rodents. A salient feature of the methionine restriction phenotype is the significant reduction of adipose tissue mass, which is associated with improvement of insulin sensitivity. These beneficial effects of MR involve a host of metabolic adaptations leading to increased mitochondrial biogenesis and function, elevated energy expenditure, changes of lipid and carbohydrate homeostasis, and decreased oxidative damage and inflammation.

Effects of monopolar radiofrequency treatment over soft-tissue fillers in an animal model.

Background And Objectives: Monopolar radiofrequency (RF) treatment is used by physicians to tighten and contour the skin of their patients. In many cases, patients have received prior treatment with other aesthetic modalities such as soft-tissue augmentation or they may wish to receive these treatment modalities simultaneously. Together, soft-tissue augmentation and monopolar RF treatment have the potential to restore tissue volume and improve facial laxity.

Highly purified 1000-cSt silicone oil for treatment of human immunodeficiency virus-associated facial lipoatrophy: an open pilot trial.

Background: Among human immunodeficiency virus-infected individuals, facial lipoatrophy has become epidemic. Those affected are stigmatized, leading to psychological distress, social and career impediments, and impaired compliance to human immunodeficiency virus medications. Temporary treatment options are limited by excessive cost, necessity of frequent treatments, and lack of a natural look or feel beneath the skin.

A case of HIV-associated facial lipoatrophy treated with 1000-cs liquid injectable silicone.

Background: Patients infected with the HIV may experience HIV-associated lipodystrophy, a symptom of which is facial lipoatrophy, a dramatic loss of subcutaneous facial tissue. This visible manifestation of HIV infection causes significant psychosocial pain for patients and has been associated with impaired compliance with medical regimens.

Objective: To improve the appearance of facial lipoatrophy in a safe, long-lasting, expeditious, and relatively economical manner.

Liquid injectable silicone: techniques for soft tissue augmentation.

This article includes a description of the uses and indications of liquid injectable silicone and a discussion of the results obtained. Patient selection, indications, mechanism of action, potential complications, and adjunct procedures are also addressed.

Dermabrasion. As a complement to dermatology.

This article includes a description of the method of dermabrasion and a discussion of the results obtained in various types of dermatologic conditions. Indications for surgery, appropriate patient selection, adjunctive surgical procedures, potential complications, comparative modalities, and some recent controversies also are addressed.

Subcutaneous incisionless (subcision) surgery for the correction of depressed scars and wrinkles.

Background: A new method of subcuticular underming for the treatment of depressed cutaneous scars and wrinkles is introduced.

Objective: To define the newly coined term "Subcision" and to describe this minor surgical procedure for treating depressed scars and wrinkles.

Methods: A tri-beveled hypodermic needle is inserted through a puncture in the skin surface (hence, "incisionless" surgery), and its sharp edges are maneuvered under the defect to make subcuticular cuts or "-cisions.

Dermabrasion.

This article describes the method of dermabrasion and results obtained in various types of dermatologic conditions. Indications for surgery, appropriate patient selection, adjunctive surgical procedures, potential complications, and recent controversies are also addressed.

Granulocytic sarcoma presenting as a solitary nodule of skin in a patient with Waldenstrom's macroglobulinemia. An immunohistochemical and electron-microscopic study.

While he was being treated for Waldenstrom's macroglobulinemia, a 75-year-old man developed an enlarging forearm skin nodule. On biopsy, the lesion appeared to be a malignant lymphoma. The tumor cells were negative for immunoglobulins but positive for lysozyme and alpha-1-antitrypsin.

Acne scar revision update.

The typical patient with acne scarring has a variety of scar types. This article covers the choice and implementation of the several different techniques usually required to achieve optimal reconstruction.

Hair transplantation.

The history of hair transplantation and its recent developments are reviewed. The procedures, risks and benefits, avoidance of complications, and treatment of sequelae are discussed.