A multifunctional organelle coordinates phagocytosis and chlorophagy in a marine eukaryote phytoplankton Scyphosphaera apsteinii.

Mixotrophy via phagocytosis can have profound consequences for the survival of marine phytoplankton and the efficiency of carbon transfer in marine systems. Little is known about the cellular mechanisms that underly nutrient acquisition via prey uptake and processing in mixotrophic phytoplankton. We used confocal microscopy, flow cytometry, and electron microscopy to assess phagocytosis and intracellular prey processing in the diploid calcifying coccolithophore Scyphosphaera apsteinii.

Multi-pass, single-molecule nanopore reading of long protein strands.

The ability to sequence single protein molecules in their native, full-length form would enable a more comprehensive understanding of proteomic diversity. Current technologies, however, are limited in achieving this goal. Here, we establish a method for the long-range, single-molecule reading of intact protein strands on a commercial nanopore sensor array.