[RNA content of cells isolated from normal and arteriosclerotic human aorta].

Cells from the intimal and medial layers of the normal and atherosclerotic human aortas were isolated by enzymatic dispersion. The isolated cells were stained in suspension by intravital staining with acridine orange as fluorescent stain for nucleic acids. The intensity of red fluorescence in each cell corresponding to the RNA content was measured and recorded by flow cytofluorometer.

[Restriction mapping of plasmid pSa1, isolated from Streptomyces albus G strain].

A novel plasmid designated pSa1 has been isolated from Streptomyces albus G strain producing SalGI restriction endonuclease. Molecular weight of the plasmid is 3.4 +/- 0.

[Polymorphism of human aortic cells in culture].

A method is suggested for isolation and cultivation of cells from the intima and media of human aorta. In primary cell cultures obtained by this method, cell polymorphism has been found. Four main morphologic types of cells have been described: prolonged, asymmetric, polygonal and star-shaped.

[Identification of the restriction and modification systems in Streptomyces strains].

Host range of actinophage phi C31, VP5 and Pg81 in respect to 109 strains of Streptomyces genus and hybrid strain Rcg2 from the cross S. coelicolor A3(2)XS. griseus Kr was studied.

[Post-radiation disorders in the cyclic AMP system of mouse spleen and thymus lymphoid cells].

Early alterations in the enzyme activities controlling cyclic adenosine-3',5'-monophosphate (cAMP) metabolism after the irradiation (800 rad) of mice were found in the lymphoid cells of the spleen and thymus. Postradiation disturbances in these activities' ration induced alterations in cAMP steady-state concentrations in the cell. It was also demonstrated that irradiation reduced lymphoid cell ability to accumulate cAMP in response to isoproterenol administration.