Phenolic Compounds, Antioxidant Activity, Sensorial Analysis and Metabolic Syndrome Enzyme Inhibition Properties of Calafate (Berberis microphylla) Fruit Juice Fermented by Patagonian Lactic Acid Bacteria.

Calafate is a native barberry of Patagonia, used in culinary and medicinal practices since ancient times. The aim of this work was to analyze the ability of lactic acid bacteria (LAB) isolated from calafate fruits and flowers, to increase the phenolic compound concentration and antioxidant capacity as well as to inhibit metabolic-related enzymes in fermented calafate juices. The sensory attributes of the selected fermented juice were also analyzed.

An Actinide Complex with a Nucleophilic Allenylidene Ligand.

The reaction of [CpTh(3,3-diphenylcyclopropenyl)] (Cp = η-CH) with 1 equiv of lithium diisopropylamide (LDA) results in cyclopropenyl ring opening and formation of the thorium allenylidene complex, [Li(EtO)][CpTh(CCCPh)] ([Li(EtO)][]), in good yield. Additionally, deprotonation of [CpTh(3,3-diphenylcyclopropenyl)] with 1 equiv of LDA, in the presence of 12-crown-4 or 2.2.

Fishes (Actinopterygii) of the rapids and associated environments in the lower Vaupés River Basin: an undiscovered Colombian Amazon diversity.

The Vaupés River stands out as one of the few within the Amazon basin due to its numerous rapids. These riverine fast-flowing sections not only provide habitat to highly specialized fishes but also function as natural barriers hindering the movement of fish along its course. During a fish-collecting expedition in the lower Vaupés River basin in Colombia, 95 species were registered belonging to 30 families and seven orders.

Quantifying Actinide-Carbon Bond Covalency in a Uranyl-Aryl Complex Utilizing Solution C NMR Spectroscopy.

Reaction of [UOCl(THF)] with generated LiFmes (FmesH = 1,3,5-(CF)CH) in EtO resulted in the formation of the uranyl aryl complexes [Li(THF)][UO(Fmes)] ([Li(THF)][]) and [Li(EtO)(THF)][UO(Fmes)] ([Li(EtO)(THF)][]) in good to moderate yields after crystallization from hexanes and EtO, respectively. Both complexes were characterized by X-ray crystallography and NMR spectroscopy. DFT calculations reveal that the C resonance in [] exhibits a deshielding of 51 ppm from spin-orbit coupling effects originating at uranium, which indicates an appreciable covalency in the U-C bonding interaction.

Exiguobacterium sp. as a bioinoculant for plant-growth promotion and Selenium biofortification strategies in horticultural plants.

Plant growth-promoting rhizobacteria (PGPR) have a positive effect on plant development and being a promising way to enhance crop productivity and as substitution of chemical fertilizers. Selenium (Se) is an important trace element and its intake is usually lower than the daily minimum amount required for humans; hence, there is a demand on the design of Se biofortification strategies. Here, the genetic traits known to be associated with Plant-Growth Promotion (PGP) and Se biotransformation of Exiguobacterium sp.

Biogenic selenium nanoparticles with antifungal activity against the wood-rotting fungus .

Selenium nanoparticles (SeNPs) have antimicrobial and antifungal activity. SeNPs using Se resistant bacteria is a low cost and eco-friendly technology. Fungal contamination of wood during drying is one of the main causes of economic losses in the wood industry.

Assessing the 4f Orbital Participation in the Ln-C Bonds of [Li(THF)][Ln(CCl)] (Ln = La, Ce).

The reaction of [Ln(NO)(THF)] (Ln = La, Ce) with 4 equiv of LiCCl in EtO resulted in the formation of the homoleptic lanthanide-aryl "ate" complexes [Li(THF)][La(CCl)] ([Li][]) and [Li(THF)][Ce(CCl)] ([Li][]). These complexes represent the first isolated homoleptic perchlorophenyl complexes for the lanthanides. In the solid state, both [Li][] and [Li][] exhibit octa-coordinate lanthanide centers, with four Ln-C σ-bonds and four Cl → Ln dative interactions involving the -Cl atoms of the CCl ligands.

Homoleptic Perchlorophenyl "Ate" Complexes of Thorium(IV) and Uranium(IV).

The reaction of AnCl(DME) (An = Th, = 2; U, = 0) with 5 equiv of LiCCl in EtO resulted in the formation of homoleptic actinide-aryl "ate" complexes [Li(DME)(EtO)][Li(DME)][Th(CCl)] ([Li][]) and [Li(EtO)][U(CCl)] ([Li][]). Similarly, the reaction of AnCl(DME) (An = Th, = 2; U, = 0) with 3 equiv of LiCCl in EtO resulted in the formation of heteroleptic actinide-aryl "ate" complexes [Li(DME)(EtO)][Li(EtO)][ThCl(CCl)] ([Li][]) and [Li(EtO)][UCl(CCl)] ([Li][]). Density functional calculations show that the An-C σ-bonds are considerably more covalent for the uranium complexes vs the thorium analogues, in line with past results.

Synthesis and Characterization of Two Uranyl-Aryl "Ate" Complexes.

Reaction of [UO Cl (THF) ] with 3 equivalents of LiC Cl in Et O resulted in the formation of first uranyl aryl complex [Li(Et O) (THF)][UO (C Cl ) ] ([Li][1]) in good yields. Subsequent dissolution of [Li][1] in THF resulted in conversion into [Li(THF) ][UO (C Cl ) (THF)] ([Li][2]), also in good yields. DFT calculations reveal that the U-C bonds in [Li][1] and [Li][2] exhibit appreciable covalency.

Polyextremophilic Bacteria from High Altitude Andean Lakes: Arsenic Resistance Profiles and Biofilm Production.

High levels of arsenic present in the High Altitude Andean Lakes (HAALs) ecosystems selected arsenic-resistant microbial communities which are of novel interest to study adaptations mechanisms potentially useful in bioremediation processes. We herein performed a detailed characterization of the arsenic tolerance profiles and the biofilm production of two HAAL polyextremophiles, sp. Ver3 (Ver3) and sp.

Burnout and posttraumatic stress in paediatric critical care personnel: Prediction from resilience and coping styles.

Introduction: Our aims were (1) to explore the prevalence of burnout syndrome (BOS) and posttraumatic stress disorder (PTSD) in a sample of Spanish staff working in the paediatric intensive care unit (PICU) and compare these rates with a sample of general paediatric staff and (2) to explore how resilience, coping strategies, and professional and demographic variables influence BOS and PTSD.

Materials And Methods: This is a multicentre, cross-sectional study. Data were collected in the PICU and in other paediatric wards of nine hospitals.

An Exploratory Study of Sevoflurane as an Alternative for Difficult Sedation in Critically Ill Children.

Objectives: To analyze the effectiveness of inhaled sevoflurane in critically ill children with challenging sedation.

Design: Prospective case series.

Setting: Two PICUs of university hospitals in Spain.

Haloarchaea from the Andean Puna: Biological Role in the Energy Metabolism of Arsenic.

Biofilms, microbial mats, and microbialites dwell under highly limiting conditions (high salinity, extreme aridity, pH, and elevated arsenic concentration) in the Andean Puna. Only recent pioneering studies have described the microbial diversity of different Altiplano lakes and revealed their unexpectedly diverse microbial communities. Arsenic metabolism is proposed to be an ancient mechanism to obtain energy by microorganisms.

The genomic sequence of str. N139 reveals a species that thrives in cold waters and extreme environmental conditions.

We report the genome sequence of str. N139, isolated from a high-altitude Andean lake. Comparative genomic analyses of the genomes available suggest that our strain belongs to the same species as the previously reported str.

Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis.

Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high arsenic content, among others.

Sevoflurane Therapy for Severe Refractory Bronchospasm in Children.

Objectives: To describe the effect of inhaled sevoflurane in the treatment of severe refractory bronchospasm in children.

Design: Retrospective case series.

Setting: Two PICUs of tertiary general university hospitals in Spain.

High-Up: A Remote Reservoir of Microbial Extremophiles in Central Andean Wetlands.

The Central Andes region displays unexplored ecosystems of shallow lakes and salt flats at mean altitudes of 3700 m. Being isolated and hostile, these so-called "High-Altitude Andean Lakes" (HAAL) are pristine and have been exposed to little human influence. HAAL proved to be a rich source of microbes showing interesting adaptations to life in extreme settings (poly-extremophiles) such as alkalinity, high concentrations of arsenic and dissolved salts, intense dryness, large daily ambient thermal amplitude, and extreme solar radiation levels.

Draft Genome Sequence of the Polyextremophilic Exiguobacterium sp. Strain S17, Isolated from Hyperarsenic Lakes in the Argentinian Puna.

Exiguobacterium sp. strain S17 is a moderately halotolerant, arsenic-resistant bacterium that was isolated from Laguna Socompa stromatolites in the Argentinian Puna. The draft genome sequence suggests potent enzyme candidates that are essential for survival under multiple environmental extreme conditions, such as high levels of UV radiation, elevated salinity, and the presence of critical arsenic concentrations.

Proteomic approach of adaptive response to arsenic stress in Exiguobacterium sp. S17, an extremophile strain isolated from a high-altitude Andean Lake stromatolite.

The North-Western part of Argentina is particularly rich in wetlands located in the Puna in an altitude between 3,600 and 4,600 m above sea level. Most of these high-altitude Andean lakes are inhospitable areas due to extreme habitat conditions such as high contents of toxic elements, particularly arsenic. Exiguobacterium sp.

Lipid storage in high-altitude Andean Lakes extremophiles and its mobilization under stress conditions in Rhodococcus sp. A5, a UV-resistant actinobacterium.

The production of triacylglycerols (TAG) or wax esters (WS) seems to be a widespread feature among extremophile bacteria living in high-altitude Andean Lakes (HAAL), Argentina. Twelve out of twenty bacterial strains isolated from HAAL were able to produce TAG or WS (between 2 and 17 % of cellular dry weight) under nitrogen-limiting culture conditions. Among these strains, the extremophile Rhodococcus sp.

Use of combined transmucosal fentanyl, nitrous oxide, and hematoma block for fracture reduction in a pediatric emergency department.

Objective: The objective of this study was to evaluate the use of combined inhaled nitrous oxide (NO), hematoma block (HB), and transmucosal fentanyl (TMF) as sedoanalgesia in the reduction of radioulnar fractures in children in a pediatric emergency department (PED).

Methods: A retrospective, analytical observational study examining the cases of radioulnar fracture reduction in PED from 2007 to 2009 in children from 4 to 15 years old. The cases were divided into 2 groups: those in which only NO + HB was used and those in which TMF was combined with NO + HB.

Genome sequence of Sphingomonas sp. S17, isolated from an alkaline, hyperarsenic, and hypersaline volcano-associated lake at high altitude in the Argentinean Puna.

The high-altitude Andean lakes (HAAL) in the Argentinean Puna-high Andes region represent an almost unexplored ecosystem exposed to extreme conditions (high UV irradiation, hypersalinity, drastic temperature changes, desiccation, and high pH). Here we present the first genome sequence, a Sphingomonas sp., isolated from this extreme environment.