Publications by authors named "Orain N"
Article Synopsis
- Treponematoses are chronic bacterial diseases primarily transmitted through sexual contact or direct interaction, but their epidemiology in wild primates remains largely unexplored due to the difficulty of studying these animals.
- This study focused on critically endangered Western chimpanzees in Senegal, using a non-invasive stool serology method that revealed anti-treponemal antibodies in 13 out of 29 chimpanzees tested.
- The findings highlight the importance of using non-invasive methods for disease surveillance in wildlife, emphasizing the need to assess and manage the risk of treponematosis within chimpanzee populations as part of a comprehensive One Health strategy.
Objective: Vanishing viral RNA restricts our ability to detect ancient pathogens, so, we used paleo serological approaches to trace the dynamics of the Coronavirus in ancient populations.
Materials And Methods: We investigated 10 ancient dental calculus samples collected from a cemetery dated to the beginning of the 19th century and excavated in Charleville-Mézières. After paleoserum samples were extracted from dental calculus, paleoserology using mini-line-blot incorporating one alpha-Coronavirus (Coronavirus 229 E) and two beta-Coronavirus (Coronavirus OC 43, SARS-CoV-2) antigens and controls was completed by an automated Western blotting assay.
Article Synopsis
- Kidney transplant recipients (KTRs) remain at high risk for severe COVID-19, necessitating booster vaccinations and monoclonal antibody treatments for better protection.
- A study found that only 46% of vaccinated KTRs could neutralize various SARS-CoV-2 variants, with many showing poor response to Omicron variants.
- Tixagevimab/cilgavimab showed limited effectiveness, especially against newer Omicron strains, highlighting the need for improved strategies for protecting KTRs from COVID-19.
Evidence of antibodies against SARS-CoV-2 in wild mustelids from Brittany (France).
Transbound Emerg Dis
September 2022
In the French region of Brittany, mainly in the department of the Côtes d'Armor, during the first half of 2021, seropositivity for SARS-CoV-2 was detected in five wild mustelids out of 33 animals tested (15.6%). Anti-SARS-CoV-2 IgG was detected against at least four out of five recombinant viral proteins (S1 receptor binding domain, nucleocapsid, S1 subunit, S2 subunit and spike) in three pine martens (Martes martes) and in two badgers (Meles meles) using the automated western blot technique.
View Article and Find Full Text PDFBackground: The incidence of poliovirus has been significantly reduced by as much as 99.9% globally. Alongside this, however, vaccine-associated paralytic poliomyelitis has emerged.
View Article and Find Full Text PDFAutomated Western immunoblotting detection of anti-SARS-CoV-2 serum antibodies.
Eur J Clin Microbiol Infect Dis
June 2021
ELISA and chemiluminescence serological assays for COVID-19 are currently incorporating only one or two SARS-CoV-2 antigens. We developed an automated Western immunoblotting as a complementary serologic assay for COVID-19. The Jess Simple Western system, an automated capillary-based assay, was used, incorporating an inactivated SARS-CoV-2 lineage 20a strain as the source of antigen, and total immunoglobulins (IgG, IgM, IgA) detection.
View Article and Find Full Text PDFIntroduction: The ECCA grading scale (échelle d'évaluation clinique des cicatrices d'acné) is a tool designed to help dermatologists to assess the severity of acne scars and to standardize the discussions about the treatments of scars.
Methods: We developed an acne scar clinical grading scale called ECCA, which consists of 6 items designed to assess easily and quickly the severity of acne scars by a global score. The interobserver reliability of the ECCA grading was statistically validated.