Morphological Changes in Tissue When Using Polypropylene Implants with Adsorbed Multipotent Stromal Cells in Experiment.

The subcutaneous tissue of rats after implantation of polypropylene materials with adsorbed bone marrow-derived mesenchymal multipotent stromal cells (MMSCs) was studied using light microscopy. Inflammation in response to implantation was mild, and the foreign material was encapsulated into a thin strip of dense fibrous connective tissue with multinucleated macrophages. By 1 year after introduction of the monofilament and 6 and 12 months after implantation of the mesh product, some threads were deformed, broken, and had sharp edges.

[The cell technologies in modification of mesh materials used in urology].

Based on the literary analysis, the influence of cellular technologies on the results of implantation of mesh materials was studied. The scientific literature of recent years contains a large amount of data devoted to the study of mesh structures and the possibilities of their modification using multipotent stromal cells (MSC) for implantation into patients for correcting tissue defects and pelvic organ prolapse. However, the ideal implant has not yet been created.

Preservation of Mesenchymal Stem Cell-Derived Extracellular Vesicles after Abdominal Delivery in the Experiment.

Light luminescent microscopy was used to study the distribution of extracellular microvesicles with PKH26-stained membranes secreted by placenta-derived mesenchymal stromal cells in the uterine tissues at different terms after injections to intact rats and after abdominal delivery (a model of cesarian section). Microvesicles migrated through the uterine tissues and were detected for at least 8 days after injection. In some cases, microvesicles were more numerous in the uterus after cesarian section modeling, which can be related to blockade of microcirculation and lymph flow due to inflammation accompanying surgical intervention.

[Liver regeneration after resection in clinical and experimental conditions].

The researches devoted to postoperative liver regeneration and influence in this process were analyzed. Liver injury is followed by hypertrophy of residual liver parenchyma. The use of various cytokines is perspective for activation, acceleration and inhibition of liver recovery.

[ROLE OF VARIOUS ANTIGENIC PREPARATIONS OF FRANCISELLA TULARENSIS IN FORMATION OF ALLERGY REACTION IN HUMANS AND ANIMALS].

Aim: Study the role of LPS in induction of anti-tularemia immunity in humans and animals.

Materials And Methods: Activity of various antigenic preparations of tularemia microbe, including highly purified from protein and S- and R-LPS, was studied using leukocytolysis reaction with blood of vaccinated humans and guinea pigs and skin allergy test (guinea pigs).

Results: Only the whole cells of Francisella tularensis, killed in protein non-denaturating conditions and conserving full S-LPS structure (tularin⁺) were shown to be inductors of delayed-type hypersensitivity reaction.

Possibility of Using Mesenchymal Stromal Cells to Restore Lymph Flow in Experimental Phlebothrombosis.

The possibility of formation of lymphatic vessels after introduction of autologous bone marrow-derived multipotent mesenchymal stromal cells transfected with GFP gene into thrombosed femoral vein was studied by fluorescent microscopy. Vascular thrombosis caused by ligation of the great vein with subsequent injection of thrombin solution was accompanied by blockade of regional lymph flow. The cells injected into thrombosed vein directly participate in the formation of new lymphatic vessels in the paravasal tissue surrounding the vein, its tissue region, and around regional lymph nodes.

Morphological Changes in Rat Uterine Tissues and Possibility of Spontaneous Labor as a Result of Injection of Multipotent Mesenchymal Stromal Cells against the Background of Hydrometra.

The possibility of pregnancy and labor was evaluated and tissue changes after injection of autologous bone marrow multipotent mesenchymal stromal cells with transfected GFP gene were studied in rats with experimental hydrometra. Injection of stromal cells to the uterine cicatrix increased the number of vessels (vascular walls or their elements) formed de novo with participation of injected cells. The animals produced progeny 2 estrous cycles earlier, the percentage of "puerperal" rats in this group was higher, their progeny was more numerous and they had the maximum numbers of little rats.

[The rat submandibular lymph node after introduction in mandibular bone defect ultipotent mesenchymal cells adsorbed on polyhydroxyalkanoate scaffold].

The reactions of rat regional lymph nodes, caused by implantation of the autologous multipotent mesenchymal stromal cells of a bone marrow origin (AMMSCBM) for acceleration of bone defect regeneration in bottom jaw experiment were studied by methods of fluorescent light microscopy. After introduction in an injury site of a bottom jaw bone of polyhydroxyalkanoate with adsorbed AMMSCBM with a transfected GFP gene the numerous large macrophages with a set of oval fluorescent inclusions in cytoplasm appear in lymph nodules of submandibular lymph nodes. The number of such macrophages increases within 2 weeks after operation, and further starts decreasing.

[Comparative analysis of parameters of humoral and cell specific immunity in individuals immunized with a live tularemia vaccine].

Aim: Comparative analysis of parameters of humoral and cell immunity in individuals, vaccinated against tularemia.

Materials And Methods: Sera and blood samples of 258 immunized individuals were studied by indirect hemagglutination and leukocytolis with tularin reaction.

Results: 73% of the examined individuals had both specific antibodies and positive values of cell immunity.

[Interaction of S- and R-lipopolysaccharides of Francisella tularensis with lypopolysaccharide-binding protein of human serum].

Investigation of ability of Francisella tularensis S- and R-lypopolysaccharide (LPS) preparations as well as the live bacteria with different chemotypes to interact with human lypopolysaccharide-binding protein (LBP) was carried out. It was found that LPS preparations derived from virulent(S-LPS) or isogenic avirulent mutant (R-LPS) strains of F. tularensis had markedly lower affinity to LBP as compared with typical S-LPS of Salmonella abortus and R-LPS of Yersinia pestis.

[The role of lipopolysaccharide in toxicity of Francisella genus bacteria].

It was demonstrated that the lipopolysaccharides (LPS) preparations, which were isolated from all representatives of Francisella Genus bacteria, i.e. F.

[Species- and genus-specific antigenic epitopes of Francisella tularensis lipopolysaccharides].

Lipopolysaccharide (LPS) antigenic epitopes of natural virulent and isogenic avirulent Francisella tularensis strains and other species of the Francisella genus (F. novicida, F. novicida-like, and F.

[Dynamics of the development of disseminated intravascular coagulation syndrome in dystocia].

A comprehensive investigation of the hemostasis system permitted to follow the dynamics of disseminated intravascular hemocoagulation syndrome in patients with dystocia. In patients with spastic contractility and myometrial hypertonicity hypercoagulation stage of the aforementioned syndrome was preserved, in those with uterine inertia a transitory stage persisted, those with hypotonicity demonstrated hypocoagulation stage of the syndrome development. It was concluded that the prevention of acute syndrome stages depended on the rapid recovery of coordinated uterine contractility.