Utility of culture and molecular methods using Allplex Bacterial Vaginosis Plus Assay (Seegene) as a tool for endometriosis, infertility and recurrent pregnancy loss diagnosis.

Endometriosis, infertility, or recurrent pregnancy loss (RPL) are entities characterised by a decrease in Lactobacillus spp. and an increase in bacterial vaginosis-associated bacteria, (BVAV) according with 16S rRNA sequencing studies. However, the use of nucleic acid amplification tests (NAAT) as a tool for diagnosis algorithms is unknown.

Variability between the follicular steroid hormone levels in different follicles of the same patient and between patients.

Context: There is disagreement as to whether the concentration of a substance in follicular fluid is related to the quality of the follicle as a possible reflection of the oocyte quality or whether this concentration is related to the clinical characteristics of the patient.

Aim: To establish the variability of steroid hormone levels in follicular fluids from different follicles of the same patient and between patients.

Settings And Design: Prospective cohort study.

Follicular steroid hormones as markers of oocyte quality and oocyte development potential.

Context: Various components of follicular fluid are suggested as biochemical predictors of oocyte quality. Previous studies of follicular steroid hormone levels have shown disparate results when related with fertilization outcomes.

Aim: The objective of the study was to relate the levels of steroid hormones of each individual follicle with oocyte maturation, fertilization results, embryo quality, and pregnancy rates.