In vitro evaluation of the effect of the electronic cigarette aerosol, Cannabis smoke, and conventional cigarette smoke on the properties of gingival fibroblasts/gingival mesenchymal stem cells.

Objective: The current study aimed to evaluate the effect of electronic cigarette (EC) aerosol, Cannabis, and conventional cigarettes smoke on gingival fibroblast/gingival mesenchymal stem cells' (GF/G-MSCs) of never smokers.

Material And Methods: Human GF/G-MSCs (n = 32) were isolated and characterized using light microscopy, flow cytometry, and multilineage differentiation ability. Following the application of aerosol/smoke extracts, GF/G-MSCs were evaluated for cellular proliferation; colony-forming units (CFU-F) ability; cellular viability (using the MTT assay); mitochondrial depolarization using JC-1 dye; and genes' expression of ATM, p21, Oct4, and Nanog.

Anterior Maxilla Augmentation Using Palatal Bone Block with Platelet-Rich Fibrin: A Controlled Trial.

Purpose: This study was conducted to characterize clinically and radiographically the effect of using platelet-rich fibrin (PRF) autologous graft on the augmentation results of autogenous palatal bone blocks.

Materials And Methods: Patients who suffered from horizontal alveolar bone defects in the anterior maxillary ridge (≤ 4.5 mm) with one or two missing teeth were augmented with autogenous palatal bone blocks 4 months prior to implant placement.