Semi-automated red blood cell core detection in blood micro-flow.

In microcirculation, red blood cells (RBCs) tend to migrate toward the centre of the vessel leaving a region of a cell depleted layer or cell-free layer (CFL) at the vessel wall and a core of RBCs at the centre. This heterogenous distribution of cells has an effect on the blood apparent viscosity and the exchanges of gases and nutrients between the RBCs and the vessel. Understanding the formation of the CFL and obtaining accurate measurement of it is paramount for furthering development of devices such as drug administration.