Green synthesis of silver nanoparticles mediated L. (Persimmon): determination of chemical composition and evaluation of their antimicrobials and anticancer activities.

The eco-friendly synthesis of metallic nanoparticles (MNPs) using biological materials is an encouraging and innovativeness approach to nanotechnology. Among other synthesizing methods, biological methods are chosen because of their high efficiency and purity in many aspects. In this work, using the aqueous extract obtained from the green leaves of the L.

The Investigation of the Chemical Composition and Applicability of Gold Nanoparticles Synthesized with (Almond) Leaf Aqueous Extract as Antimicrobial and Anticancer Agents.

The current work's main objective was to determine the chemical composition of (AC) leaf extract and examine the antibacterial and cytotoxic properties of biosynthesized gold nanoparticles (AuNPs). The chemical composition of AC leaf extract was determined using LC-ESI/MS/MS to detect compounds that may be responsible for the reducing, stabilizing, and capping steps in the synthesis of nanoparticles and their biological activities. The AC-AuNPs were spherical, with a particle size lower than 100 nm and a face-centered cubic structure.

Intestinal Barrier in Post- Irritable Bowel Syndrome.

Background: () is one of the most common causes of bacterial gastroenteritis worldwide. One sequela of this infection is the development of post-infectious irritable bowel syndrome (PI-IBS). It has been suggested that a dysfunctional intestinal barrier may promote IBS development.

Green synthesis of silver nanoparticles based on the Raphanus sativus leaf aqueous extract and their toxicological/microbiological activities.

Silver nanoparticles (AgNPs) have several uses. Many scientists are working on producing AgNPs from plant extracts for use as biomedicines against drug-resistant bacteria and malignant cell lines. In the current study, plant-based AgNPs were synthesized using Raphanus sativus L.

Analysis of the Brucella suis Twin Arginine Translocation System and Its Substrates Shows That It Is Essential for Viability.

Bacteria use the twin arginine translocator (Tat) system to export folded proteins from the cytosol to the bacterial envelope or to the extracellular environment. As with most Gram-negative bacteria, the Tat system of the zoonotic pathogen Brucella spp. is encoded by a three-gene operon, Our attempts, using several different strategies, to create a Brucella suis strain 1330 mutant were all unsuccessful.

Genital Herpes Simplex Virus-An Updated Review.

The herpes virus was named by the Greek physician Hippocrates who called it herpes because the lesions appeared near each other and were vesicular. Alphaherpesvirinae, Betaherpesvirinae, and Gammaherpesvirinae are subfamilies of the human herpes virus family. The Alphaherpesvirinae subfamily includes the simplex viruses-HSV-1 and HSV-2-and varicellovirus-varicella zoster virus.

APPLICATION OF THROMBOLYSIS THERAPY FOR ACUTE CEREBRAL CIRCULATION IN THE REPUBLIC OF KAZAKHSTAN, COUNTRIES OF THE FAR AND NEAR FOREIGN COUNTRIES.

In the world, stroke suffers 5.6-6 million people a year. Stroke deaths are predicted to rise to 6.

Immune and microbiological disorders in women with infectious and inflammatory pathology of the cervix.

Penetration into the female genital tract of infectious agents is associated with the cause of the onset of cervicitis. In most cases, these are sexually transmitted diseases (STDs). A high level of viral and bacterial load in the study of clinical material from women with infectious and inflammatory pathology (IVP) of the cervix affects the activity of innate immunity reactions.

Development and study of nutrient media for determining the biological properties of Listeria.

The commercial nutrient media for investigation of the biologic properties of L. monocytogenes is developed. The efficiency of the use of developed media for determination of motility and lecithinase activity of Listeria in the establishment of the isolated culture to the pathogenic species is shown.

In Vivo Comparison of Chlorine-Based Antiseptics versus Alcohol Antiseptic for Surgical Hand Antisepsis.

Despite being commonly used as effective preparation for surgical hand antisepsis, alcohol solutions have major drawbacks, such as drying effect, emergence of hand eczema, and other diseases. This study aimed to demonstrate the effectiveness of sodium hypochlorite (NaOCl) and hydrogen peroxide (HO) as antiseptic in comparison to single sodium hypochlorite and 70% ethanol. In 5-day tests, the effects of 3 antiseptics were established according to standard test methods.

[Monitoring of microbiological and immunological disorders in urogenital chlamydiosis in people of reproductive age.].

The identification and analysis of microbiological and immunological disorders in urogenital chlamydiosis of mixed etiology plays an important role in understanding the pathogenesis of the development of the chronic course of the disease with severe complications (infertility, miscarriage). Along with classical microbiological studies (isolating and studying the properties of the microbiota in mixed infections), the role of antigens of all participants of the infectious process (bacteria, viruses, fungi) in the occurrence of adequate immunological reactions (the level of interferon, γ-interferon) as indicators of the immune response.

[Syndrome dopamine dysregulation and deep brain stimulation of the subthalamic nucleus in Parkinson's disease].

Aim: Dopamine dysregulation syndrome (DDS) is a complication of the dopaminergic therapy in Parkinson's disease (PD); it is manifested as a compulsive medication use and may have negative impact on patients' social, psychological, and physical functioning. An effect of deep brain stimulation in the subthalamic nucleus (DBS STN) on DDS is not fully understood. Therefore, the degree of DDS during DBS STN in PD patients was evaluated in the study.

[Effects of bilateral deep brain stimulation in the subthalamic nucleus using two methods of target structure verification].

Aim: To evaluate the efficacy of deep brain stimulation in the subthalamic nucleus (DBS STN) in patients with Parkinson's disease (PD) using different methods of targeting according to the dynamics of motor symptoms of PD.

Material And Methods: The study involved 90 patients treated with DBS STN. In 30 cases intraoperative microelectrode recording (MER) was used.

[THE SPECTRUM AND ANTIBIOTIC RESISTANCE OF AGENTS OF NOSOCOMIAL INFECTION OF OPERATION WOUNDS AND ORGANS OF URINARY EXCRETION SYSTEM IN SURGERY PATIENTS].

In risk group of development of nosocomial infections related to medical care provision, prevailed weakened patients after various surgical interferences and cauterization. The nosocomial infections related to medical care provision develop up to 20% of patients of surgical profile (infections of soft tissues - 9.5%, intra-abdominal infections - 22% and infections of urinary tracts - 4.

Retinal and nonocular abnormalities in Cyp27a1(-/-)Cyp46a1(-/-) mice with dysfunctional metabolism of cholesterol.

Cholesterol elimination from nonhepatic cells involves metabolism to side-chain oxysterols, which serve as transport forms of cholesterol and bioactive molecules modulating a variety of cellular processes. Cholesterol metabolism is tissue specific, and its significance has not yet been established for the retina, where cytochromes P450 (CYP27A1 and CYP46A1) are the major cholesterol-metabolizing enzymes. We generated Cyp27a1(-/-)Cyp46a1(-/-) mice, which were lean and had normal serum cholesterol and glucose levels.

[The specific compound and biological characteristics of agents of nosocomial pneumonia isolated in hospital surgery departments of Makhachkala].

The nosocomial pneumonia is the most prevailing form of hospital-acquired infection. It is the leading cause of mortality among all forms of hospital-acquired infections. In the departments of resuscitation and intensive therapy nosocomial pneumonia consists more than 25% of all hospital-acquired infections.

[The evaluation of diagnostic effectiveness of algorithm of isolation and express identification of opportunistic enterobacteria using national chromogenic growth mediums].

The testing of specificity and diagnostic effectiveness was applied to new national chromogenic growth mediums. The algorithm was developed to isolate and express identify the clinically significant and sanitary demonstrative opportunistic enterobacteria using chromogenic growth mediums.

Abnormal vascularization in mouse retina with dysregulated retinal cholesterol homeostasis.

Several lines of evidence suggest a link between age-related macular degeneration and retinal cholesterol maintenance. Cytochrome P450 27A1 (CYP27A1) is a ubiquitously expressed mitochondrial sterol 27-hydroxylase that plays an important role in the metabolism of cholesterol and cholesterol-related compounds. We conducted a comprehensive ophthalmic evaluation of mice lacking CYP27A1.

Spatial distribution of the pathways of cholesterol homeostasis in human retina.

Background: The retina is a light-sensitive tissue lining the inner surface of the eye and one of the few human organs whose cholesterol maintenance is still poorly understood. Challenges in studies of the retina include its complex multicellular and multilayered structure; unique cell types and functions; and specific physico-chemical environment.

Methodology/principal Findings: We isolated specimens of the neural retina (NR) and underlying retinal pigment epithelium (RPE)/choroid from six deceased human donors and evaluated them for expression of genes and proteins representing the major pathways of cholesterol input, output and regulation.

Sample prefractionation for mass spectrometry quantification of low-abundance membrane proteins.

Use of stable isotope-labeled full-length proteins as an internal standard prior to multiple reaction monitoring (MRM) analysis enables prefractionation of the target proteins and quantification of those low-abundance proteins, which cannot be reached without biological sample enrichment. In terms of membrane proteins, this benefit can be used if a sample processing workflow allows entire solubilization of membrane proteins. We have developed a universal workflow for sample processing and enrichment by optimizing washing and solubilization conditions and implementing sample fractionation by Whole Gel Eluter.

[Antibiotic resistance of P. aeruginosa strains isolated from intensive care unit patients at the Makhachkala Traumatology Center].

Nosocomial infection is most commonly registered in the traumatology and surgery units, primarily in the intensive care unit (ICU) that is considered to be an increased risk unit owing to a large number of performed manipulations and the extensive empirical use of antibiotics. In ICU patients with nosocomial pneumonias at the Traumatology Center, nonfermentative gram-negative bacteria were isolated more frequently (51%), including P. aeruginosa (35.

[Microbiological study of sanitary feature of Perinatal Center of Makhachkala City].

Aim: Evaluation of bacterial contamination of six hospital environment of Perinatal Center of Makhachkala as part of epidemiologic surveillance for nosocomial infections.

Materials And Methods: One hundred twenty-eight air samples from different hospital units and 344 swabs from hospital equipment, instruments, and inventory were tested. Dry nutrient media manufactured by Scientific Manufacturing Organization "Pitatelnye Sredy" were used for isolation and identification of microorganisms.

[Domestic chromogenic nutrient medium for differentiation of Klebsiella].

Studies to design a dry chromogenic nutrient medium for the diferentiation of Klebsiella were under way, by detecting the intracellular Klebsiella genus-specific enzyme of human potential pathogenicity--5-aminosalicylate decarboxylase. The composition of the proposed medium that ensured its high sensitivity and improved its differentiating properties as compared with the known traditional media was worked through. Klebsiella are isolated and identified on the proposed medium in one step, which substantially reduces diagnosis time and material costs and takes some burden from microbiologists.

[Growth media for isolation, accumulation, and identification of Listeria in laboratory diagnostics of mix-infections in women with obstetric-gynecological pathology].

Isolation of Lysteria monocytogenes from clinical specimens in obstetric-gynecological pathology is often difficult owing to the presence of the mixed microbial flora. Results of study of new Russian growth media for isolation of Listeria for microbiological diagnostics of listeriosis in pregnancy are presented. They show diagnostic efficacy of the developed media for laboratory diagnostics of mix-infections in women with obstetric-gynecological pathology.

[Multiple antibiotic resistance of associative microflora during urogenital pathology].

Susceptibility of associative microflora isolated from patients with inflammatory diseases of urogenital tract was investigated. Etiologic structure of the diseases and cross-resistance to antibiotics of Escherichia coli, Staphylococcusaureus, and Klebsiella pneumoniae strains isolated from women with endocervicitis and men with urethritiswas assessed. Ciprofloxacin and gentamycin had the highest activity, whereas beta-lactam antibiotics were mildly active.