Effects of formalin fixation on polarimetric properties of brain tissue: fresh or fixed?

Significance: Imaging Mueller polarimetry (IMP) appears as a promising technique for real-time delineation of healthy and neoplastic tissue during neurosurgery. The training of machine learning algorithms used for the image post-processing requires large data sets typically derived from the measurements of formalin-fixed brain sections. However, the success of the transfer of such algorithms from fixed to fresh brain tissue depends on the degree of alterations of polarimetric properties induced by formalin fixation (FF).

Robustness of the wide-field imaging Mueller polarimetry for brain tissue differentiation and white matter fiber tract identification in a surgery-like environment: an study.

During neurooncological surgery, the visual differentiation of healthy and diseased tissue is often challenging. Wide-field imaging Muller polarimetry (IMP) is a promising technique for tissue discrimination and in-plane brain fiber tracking in an interventional setup. However, the intraoperative implementation of IMP requires realizing imaging in the presence of remanent blood, and complex surface topography resulting from the use of an ultrasonic cavitation device.

Polarimetric visualization of healthy brain fiber tracts under adverse conditions: studies.

We suggest using the wide-field imaging Mueller polarimetry to contrast optically anisotropic fiber tracts of healthy brain white matter for the detection of brain tumor borders during neurosurgery. Our prior studies demonstrate that this polarimetric imaging modality detects correctly the in-plane orientation of brain white matter fiber tracts of a flat formalin-fixed thick brain specimen in reflection geometry [IEEE Trans. Med.

Method to calibrate a full-Stokes polarimeter based on variable retarders.

We present a calibration method for a full-Stokes polarimeter. The polarimeter uses two liquid-crystal variable retarders (LCVR) and a linear polarizer to measure the four Stokes parameters. The calibration method proposed in this paper calculates the errors in the experimental setup by fitting the experimental intensity measurements for a set of calibration samples to a theoretical polarimeter with errors.

Permitted experimental errors for optimized variable-retarder Mueller-matrix polarimeters.

An optimized Mueller-matrix polarimeter is simulated. The polarimeter is optimized by finding the configurations of the polarization state generator and polarization state analyzer that give the minimum condition number. Noise is included in the measurement of the polarimeter intensities, and the eigenvalue calibration procedure is used to reduce the errors in the final Mueller matrix.

Experimental limits for eigenvalue calibration in liquid-crystal Mueller-matrix polarimeters.

A numerical study is carried out to find the experimental conditions necessary for the eigenvalue calibration procedure to work correctly in a liquid-crystal variable-retarder-based Mueller-matrix polarimeter. Using the error between the simulated experimental Mueller matrix in a polarimeter with errors and the expected ideal Mueller matrices for four calibration samples, the maximum experimental errors are estimated for a successful eigenvalue calibration. It is found that the retarder axes' orientations have smaller permitted errors than the retardation values.

Calibration and data extraction in nonoptimized Mueller matrix polarimeters.

We present a method for calibration and data extraction for a nonoptimized Mueller matrix polarimeter. The advantage of this type of method is a reduction in measurement time for multiwavelength systems or in systems with slow response times. The calibration process requires the measurement of four known polarization devices.